Project description:We have sequenced a wild Prunus mume and constructed a reference sequence for this genome. In order to improve quality of gene models, RNA samples of five tissues (bud, leaf, root, stem, fruit) were extracted from the Prunus mume. To investigate tissue specific expression using the reference genome assembly and annotated genes, we extracted RNA samples of different tissues and conducted transcriptome sequencing and DEG analysis. Five RNA pools were created corresponding to different tissues of the Prunus mume.
Project description:We have sequenced a wild Prunus mume and constructed a reference sequence for this genome. In order to improve quality of gene models, RNA samples of five tissues (bud, leaf, root, stem, fruit) were extracted from the Prunus mume. To investigate tissue specific expression using the reference genome assembly and annotated genes, we extracted RNA samples of different tissues and conducted transcriptome sequencing and DEG analysis.
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in peach fruits (Prunus persica) in six different stages.
2025-12-11 | GSE165094 | GEO
Project description:The study on the resistance of Prunus davidiana to green peach aphid
Project description:Plant propagation requires adventitious root production, and the inability to root cuttings prevents some tree species from being widely used and propagated on a big scale. Studies on the differences in adventitious root production ability between species that are easily rooted and those that are difficult to root are few, despite the fact that many studies have been done to examine the developmental mechanisms of adventitious roots. To attempt to address this problem, we examined the transcriptomes of Populus davidiana (difficult-to-root) and Populus yunnanensis (easy-to-root) spikes at various intervals following cutting. We discovered that the number of differential genes was significantly greater in the 96- and 120-hour groups than in the 0- and 48-hour groups. The majority of growth hormone and cytokinin-related genes were up-regulated in P. yunnanensis, while they were down-regulated in P. davidiana.
Project description:Prunus subgenus Ceresus is a renowned ornamental wooden plant for its landscape applications and economic value in gardens. However, waterlogging during the rainy season in some areas cause deaths and heavy losses. Fortunately, we have found that a variety of Prunus subgenus Ceresus × Incisa 'Gotenba zakura' could generate aerial roots when suffering from heavy rains and sustainable floods. In this study, we conducted an associated analysis to explore the core regulating network of aerial root growth mechanism in Prunus subgenus Ceresus × Incisa 'Gotenba zakura' by combining phenological observations, physiological assays, and transcriptome comparisons across five separate stages. By analyzing the heatmap of DEGs and gene co-expression network (GCN), we have recognized genes that may play very critical roles under waterlogging stress. Gene network indicates that aerial roots can improve the tolerance under waterlogging by ROS degradation, endogenous hormone induction, as well as energy production. This discover provides a valid foundation of waterlogging tolerance of Prunus subgenus Ceresus, and molecular evidence for selecting promising rootstocks.