Project description:miRNA reveals the mechanism of pacific white shrimp (Litopenaeus vannamei) under acute alkalinity stress
| PRJNA1002632 | ENA
Project description:Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp (Litopenaeus vannamei) under acute alkalinity stress
Project description:World aquaculture production of the Pacific white shrimp (Litopenaeus vannamei) is estimated to account for 80% of the total shrimp produce worldwide. The global demand for shrimp has driven the industry to utilize and rely on semi-intensive and intensive shrimp systems. In the United States, Pacific white shrimp production can take place in semi-intensive earthen ponds, recirculating aquaculture systems (RAS), biofloc technology and green water. In this study, the effects of lowering dissolved oxygen conditions in outdoor green water tanks on global gene expression is examined. Tissue samples from the gill and intestine were collected for gene expression analysis via RNA sequencing. Among all comparisons, RNA sequencing revealed the up-regulation of a single gene: hydroxyacid oxidase 1 gene. The HOA1 gene was found to be 7-fold higher in the intestine sample at the medium aeration level compare to that of the high (control) level. The HAO1 gene, also known as glycolate oxidase 1 (GOX1) is a gene related to the 2-hydroxyacid oxidase enzyme that is part of the oxidoreductase family and plays a role in glyoxylate and dicarboxylate metabolism. The identification of a single differentially expressed gene across all analyzed samples suggests that Pacific white shrimp exposed to lowering dissolved oxygen set points does not induce global changes in gene expression at these levels.
Project description:The Pacific white shrimp (Litopenaeus vannamei), one of the most widely cultured shrimp species in the world, usually suffered from chilling stress in China. In order to reveal inner proteomics mechanism of chilling tolerance, we conducted a proteomic analysis on two contrasting shrimp cultivars, namely, chilling-tolerant GH2 and chilling-sensitive GH1 under normal temperature 28°C, chilling stress 16°C, and 16°C recovered to 28°C, respectively.
Project description:In this study, an integrative analysis of the gill-specific proteome at 0 h, 12 h and 36 h after alkalinity stress was performed to identify important regulators and pathways involved in alkalinity adaption of Exopalaemon carinicauda. This study reveals the first time-course, gill-specific, combined proteomic profiling associated with alkalinity adaption of E. carinicauda and provides new insights into the mechanisms underlying the molecular response to alkalinity stress in shrimp.
Project description:This study identified peptides in yellow mealworm protein extract (YMPE) and evaluated its cryoprotective effect on Pacific white shrimp under five freeze-thaw cycles, compared with distilled water (DW) and mixed phosphate solution (MP).
Project description:Vibrio parahaemolyticus is a Gram-negative bacterium commonly found in marine and estuarine environments. Acute hepatopancreatic necrosis disease (AHPND) caused by this bacterium is an ongoing problem among shrimp farming industries. V. parahaemolyticus proteins PirA and PirB have been determined to be major virulence factors that induce AHPND. In this study, Pacific white shrimp (Litopenaeus vannamei) were challenged with recombinant PirA and PirB by a reverse gavage method and then at 30 m, 1, 2, 4, and 6 h time points, the hepatopancreas of five individual shrimp were removed and placed into RNA later. We conducted RNA sequencing of the hepatopancreas samples from a no PirA/B control (n = 5) and PirA/B-treated shrimp at the different time intervals (n=5). We evaluated the different gene expression patterns between the time groups to the control with a focus on identifying differences in innate immune function.
2023-05-05 | GSE200137 | GEO
Project description:RNA-seq of Pacific white shrimp (Litopenaeus vannamei) under long-term high salinity stress
| PRJNA649598 | ENA
Project description:Pacific white shrimp 16S rDNA
| PRJNA729583 | ENA
Project description:Gut metagenome of white shrimp under antibiotics stress