Similar Datasets
Project description:Male C57Bl/6 mice were randomized to undergo 5 days of i) a shiftwork protocol (SW; 10-hour light: 10-hour dark cycle) followed by myocardial infarction (MI) surgery, or ii) a normal environment (NSW; 12-hour light: 12-hour dark cycle) followed by MI surgery, or iii) the NSW protocol without MI, or iv) the SW protocol without MI. After 5 days, the SW and NSW light:dark cycles had realigned, and all mice were placed on a normal 12-hour light:12-hour dark cycle. MI was induced by left anterior descending (LAD) coronary artery ligation at Zeitgeber Time 06 (ZT06; 6 hours after lights on). All mice were maintained on the normal 12-hour light:12-hour dark cycle thereafter. Hearts were collected at ZT06, 24 hours post-MI; hearts from the corresponding non-MI NSW and SW groups were also collected at ZT06. The microarray approach allows the investigation of transcriptome-wide gene expression changes in hearts from mice exposed to a shiftwork or normal light:dark cycle, both in the absence of MI and at 24 hours post-MI.
2018-11-30 | GSE123092 | GEO
Project description:We used single-cell sequencing data and imaging to investigate Eukaryotic plankton from environmental marine samples collected from Coogee, NSW, Australia.
2024-08-18 | GSE274796 | GEO
Project description:In plants, programmed cell death (PCD) is involved in both the development and in the response to biotic and abiotic aggressions. In early stages of PCD, mitochondrial membranes are made permeable by the formation of permeability transition pores, whose protein composition is debated. Since flooding stress can produce PCD in several plant species, the first goal of this work was to know if flooding stress could be used to induce PCD in Beta vulgaris roots. To do this, two months old beet plants were flood-stressed from one to five days, and the alterations indicating PCD in stressed beetroot cells were observed with a confocal fluorescence microscope. In addition, cytochrome c was released from mitochondria. After assessing that flood stress induced PCD in beetroots, the composition of mitochondrial protein complexes was observed in control and flood-stressed beetroots. Protein complexes from isolated mitochondria were separated by native gel electrophoresis, and their proteins were identified by mass spectrometry. The spectra count of three isoforms of voltage-dependent anion-selective channels (VDAC) increased after one day of flooding. In addition, the size of the complexes formed by VDAC was higher in beetroots flood-stressed for one day (~200 kDa) compared with non-stressed ones (~100 kDa). Other proteins, like chaperonin CPN60-2, also formed complexes with different masses in control and flood-stressed beetroots. Finally, possible interactions of VDAC with other proteins were found performing a cluster analysis. These results indicate that mitochondrial protein complexes formed by VDAC could be involved in the process of programmed cell death in flood-stressed beetroots
2021-10-28 | PXD027781 | Pride
Project description:In the present investigation, the genetic diversity architecture of 96 deep-water rice genotypes of Assam and association mapping strategy was, for the first time, applied to determine the significant SNPsand genes for deep-water rice. These genotypes are known for their unique elongation ability under deep-water condition.The internode elongation under water related genes will be identified here can provide affluent resources for rice breeding especially in flood-prone areas. We investigated the genome-wide association studies (GWAS) using 50 K rice genic SNPchip across 96 deep-water rice genotypes collected from different flood-prone districts/villages of Assam.
2025-05-01 | GSE231797 | GEO
Project description:Buttiauxella noackiae NSW 11 genome sequencing
| PRJNA546968 | ENA