Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of pyruvate (PA), lactate (LA), or 12-hydroxyeicosatetraenoic acid (12-HETE) stimulation on type1 conventional dendritic cells (cDC1) from the human ileum

ABSTRACT: Effect of pyruvate (PA), lactate (LA), or 12-hydroxyeicosatetraenoic acid (12-HETE) stimulation on type1 conventional dendritic cells (cDC1) from the human ileum

PROVIDER: PRJNA1005658 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25648974.fastq.gz	Fastqsanger.gz
	SRR25648975.fastq.gz	Fastqsanger.gz
	SRR25648976.fastq.gz	Fastqsanger.gz
	SRR25648977.fastq.gz	Fastqsanger.gz
	SRR25648978.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Effect of pyruvate (PA), lactate (LA), or 12-hydroxyeicosatetraenoic acid (12-HETE) stimulation on type1 conventional dendritic cells (cDC1) from the human ileum

Project description:We here report that GPR31 is specifically expressed in cDC1 in human intestinal mononuclear phagocytes. Since GPR31 has been previously reported to be activated by PA, LA, and 12-HETE, RNA-seq was performed to analyze the transcriptional changes in human ileal cDC1 under the stimulation of PA, LA, or 12-HETE.

2024-10-08 | GSE240895 | GEO

12-HETE suppresses CD8+ T cell effector function via p38 MAPK signaling in MASH-HCC

2026-08-26 | GSE302549 | GEO

PCK1 deficiency promotes MASH-HCC progression by 12-HETE-induced CD8+ T cell dysfunction

Project description:Metabolic dysfunction-associated steatohepatitis-related hepatocellular carcinoma (MASH-HCC) has been reported to be less effective to immune checkpoint inhibitors, which may be associated with metabolic reprogramming of tumor cells and abnormal tumor microenvironment. Here, we aim to investigate the role of gluconeogenic enzyme phosphoenolpyruvate carboxykinase 1 (PCK1) in MASH-HCC and its interplay with the tumor microenvironment. Hepatocyte-specific phosphatase and tensin homolog (Pten) and Pck1 biallelic knockout mice were established to induce MASH-HCC. Single-cell RNA sequencing and multiparametric flow cytometry were performed to analyze the immune landscape alterations. PCK1 is downregulated in tumors tissues compared with adjacent non-cancerous tissues from patients with MASH-HCC. Hepatocyte-specific Pck1 knockout mice exhibited markedly increased tumorigenesis in dietary models and genetic models of spontaneous MASH-HCC, together with inhibited effector function of tumor-infiltrating CD8+ T cells. Mechanistically, PCK1 deficiency induces the accumulation of endogenous metabolite 12-hydroxyeicosatetraenoic acid (12-HETE), which can be taken up by CD8+ T cells and activate the p38 mitogen-activated protein kinase pathway, ultimately leading to CD8+ T cells dysfunction. Notably, PCK1 restoration or 12-HETE inhibition combined with anti-PD1 treatment increases the antitumor capability of CD8+ T cells and suppresses MASH-HCC development. This study reveals the pivotal role of the hepatic cell-intrinsic enzyme PCK1 in mediating CD8+ T cell dysfunction via 12-HETE-p38 signaling in MASH-HCC. PCK1 could be a metabolic checkpoint to enhance the efficacy of anti-PD1 immunotherapy in MASH-HCC.

2026-08-26 | GSE302896 | GEO

Effect of 20-Hete and dietary restriction on murine kidneys

Project description:Transcriptomic dataset of 15 murine kidneys of 14-week-old male C57Bl6 mice. Mice were divided into 3 treatment groups four weeks prior to nephrectomy and 5 mice had ad libitum (AL) access to food and water, while another 10 mice received 30% food restriction (CR). Calorically restricted mice either received intraperitoneal supplementation of 20-HETE (HETE) or vehicle (Veh, EtOH in sodiumchloride 0.9%) for 8 days on a daily basis prior to nephrectomy. Ad libitum fed mice received vehicle injections for 8 days on a daily basis prior to nephrectomy.

2022-05-10 | E-MTAB-11428 | biostudies-arrayexpress

PCK1 deficiency promotes MASH-HCC progression by 12-HETE-induced CD8+ T cell dysfunction

Project description:PCK1 deficiency promotes MASH-HCC progression by 12-HETE-induced CD8+ T cell dysfunction

| PRJNA1292780 | ENA

RNA-seq of Zbtb10 Knockdown and Control cDC1 dendritic cells (Cd8+)

Project description:RNA-seq of the immune-suppressed cDC1 was done to look into the mechanism underlying TLR9. It was then compared with the inflammatory cDC1 DCs.

2021-09-03 | E-MTAB-9495 | biostudies-arrayexpress

Expression Profiles of 15(S)-HETE treated RAW 264.7 cells

Project description:Hemozoin phagocytosis results in immunomodulation. This study was designed to explore gene expression responses to 15(S)-HETE in LPS-stimulated RAW 264.7 cells.

2009-12-17 | GSE15070 | GEO

ZEB1 ChIP-seq Data from Control CD8+cDC1

Project description:To identify the direct targets of Zeb1 we performed ChIP-seq of wild type cDC1 cell line in unstimulated condition. cDC1 cell line was used for Chromatin Immunoprecipitation, it was then fixed and crosslinked and then fragmented and the fragmented DNA-protein was immunoprecipated using Zeb1 antibody. The chromatin sample was then used to prepare library using NEB kit following the manufacturer's protocol

2018-11-13 | E-MTAB-6917 | biostudies-arrayexpress

Early life provision of 12-HETE by neutrophils imprints self-renewing capacity on alveolar macrophages

Project description:Early life provision of 12-HETE by neutrophils imprints self-renewing capacity on alveolar macrophages

| PRJNA894158 | ENA

12-HETE suppresses CD8+ T cell effector function via p38 MAPK signaling in MASH-HCC

Project description:12-HETE suppresses CD8+ T cell effector function via p38 MAPK signaling in MASH-HCC

| PRJNA1291167 | ENA