Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1005949 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:<p>Background The intestinal microbiota, metabolites, and quorum-sensing (QS) functions of laying hens under different proportions of fermented Cordyceps militaris mushroom substrate (CMMS) replacement diets are critical for their production performance and health. However, the changes in the intestinal microbiota, metabolites, and QS functions of laying hens under different proportions of fermented CMMS replacement diets, as well as their effects on laying hen production performance, remain unclear. This study evaluated the changes in production performance, apparent digestibility, digestive enzyme activity, serum immunoglobulin levels, cecal microbiota and metabolites, and QS function of laying hens under different proportions of fermented CMMS replacement diets, as well as their impact on the health of laying hens.</p><p>Results Using Hymenoptera brown egg-laying chicken as the research subject, this study aimed to determine the production performance, egg quality, apparent digestibility, digestive enzyme activity, and serum immunoglobulin levels of egg-laying chickens under different ratios of fermented CMMS replacement diets. Additionally, the study analyzed the cecal microbiome and metabolome. The dry matter, neutral detergent fiber, and phosphorus content of CMMS significantly decreased after fermentation treatment (P < 0.05). When 30% of the diet was replaced with fermented CMMS, the laying rate, average egg weight, average daily feed intake, and feed-egg ratio significantly increased (P < 0.05). Egg yolk color and Haugh unit significantly increased (P < 0.05), and lipase significantly increased (P < 0.05). When 20% of the diet was replaced with fermented CMMS, the apparent digestibility of DM, CP, ash, and NDF in laying hens significantly increased (P < 0.05), and IgA and IgM significantly increased (P < 0.05). Metagenome analysis showed that when fermented CMMS replaced 30% of the diet, the relative abundance of Phocaeicola, Alistipes, and Parabacteroides significantly increased (P < 0.05), Kyoto Encyclopedia of Genes and Genomes (KEGG) level 2 pathway energy metabolism increased, and QS pseudouridine-synthase RluA family genes increased. A total of 1,529 differentially expressed metabolites were identified under different proportions of fermented CMMS replacement diets. The highest relative content was found in carboxylic acids and derivatives (21.20%), and taurine and hypotaurine metabolism and glycosylphosphatidylinositol (GPI)-anchor biosynthesis were enhanced. When fermented CMMS replaced 30% of the diet, the relative abundance of Parabacteroides significantly increased (P < 0.05) and was significantly negatively correlated with tetradecanedioic acid and sebacic acid (r < 0, P < 0.001). The relative abundance of Alistipes significantly increased (P < 0.05) and was significantly positively correlated with the PemK/MazF family (r > 0, P < 0.001).</p><p>Conclusions The relationship between the microbial community in the cecum of laying hens, its metabolites, and QS function under different proportions of fermented CMMS replacement diets, as well as their regulation of production performance, apparent digestibility, and immune levels. It determined their potential impact on the metabolism and health of laying hens. The research results provide valuable insights into the potential of CMMS as an unconventional feed.</p>
2025-09-09 | MTBLS12825 | MetaboLights
Project description:<p>Background Both high fiber diet and N-Carbamylglutamate (NCG) individually contributes to improve reproductive efficiency in sow. However, the potential synergistic effects and the underlying mechanisms are not well-understood. Here, Landrace x Yorkshire sows were selected to investigate the synergistic effects of high fiber diet and 0.05% NCG on reproductive performance using metagenomic, metabolomic, and transcriptomic analysis.</p><p>Results The study revealed that high fiber with NCG (H+N) significantly increased litter weight at weaning and the apparent nutrient digestibility. Metagenomic analysis revealed H+N increased the abundance of lactobacilli across multiple taxonomic levels (from order to species level, including Lactobacillus sp. 910589175), which was positively correlated with elevated levels of the lipid metabolites L-α-glycerylphosphorylcholine and taurocholic acid. Subsequent transcriptomic profiling indicated activation of the PI3K-Akt signaling pathway, which was associated with improved litter weight at weaning and the apparent nutrient digestibility.</p><p>Conclusion The synergistic effect of high-fiber diet and NCG supplementation enhances reproductive efficiency in sows by modulating gut microbiota (specifically lactobacilli), increasing beneficial lipid metabolites, and activating the PI3K-Akt signaling pathway, thereby improving nutrient digestibility and litter weight.</p>
2025-12-16 | MTBLS13516 | MetaboLights
Project description:Effects of Dietary Addition of Ellagic Acid on Rumen Metabolism, Nutrient Apparent Digestibility, and Growth Performance in Kazakh Sheep
| PRJNA1037672 | ENA
Project description:Fermented Corn Straw Increases Cellulase Activity, Improves Rumen Fermentation, and Increases Nutrient Digestibility in Yichang White Goats (PRJCA046077)
| PRJDB39346 | ENA
Project description:Primary outcome(s): Development of an automatic bacterial flora evaluation program based on the indices obtained from comprehensive integrated analysis
| 2661190 | ecrin-mdr-crc