Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1006699 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Persistence of Listeria monocytogenes in fresh-cut produce facilities
| PRJEB111355 | ENA
Project description:This study will evaluate the safety and tolerability of a personalized live, attenuated, double-deleted Listeria monocytogenes (pLADD) treatment in adults with metastatic colorectal cancer.
| 2247134 | ecrin-mdr-crc
Project description:Listeria monocytogenes is a human, food-borne pathogen. Genomic comparisons between L. monocytogenes and Listeria innocua, a closely related non-pathogenic species, were pivotal in the identification of protein coding genes essential for virulence. However, no comprehensive comparison has focused on the non-coding genome. We used strand-specific cDNA sequencing to produce genome-wide transcription start site (TSS) maps for both organisms, and developed a publicly available integrative browser to visualize and analyze both transcriptomes in different growth conditions and genetic backgrounds. Our data revealed conservation across most transcripts, but significant divergence between the species in a subset of non-coding RNAs. In L. monocytogenes we identified 113 sRNAs and 70 asRNAs, significantly increasing the repertoire of non coding RNAs in this species. Remarkably, we identified a class of long antisense transcripts (lasRNAs) that overlap one gene while also serving as the 5M-bM-^@M-^Y UTR of the adjacent divergent gene. Experimental evidence suggests that lasRNAs transcription inhibits expression of one operon while activating the expression of another. Such lasRNA/operon structure, termed "excludon", might represent a novel form of regulation in bacteria. Construction of consensus TSS-maps in Listeria monocytogenes and Listeria innocua by applying 5'-end sequencing on samples in different conditions and genetic backgrounds.
2012-05-01 | E-GEOD-36060 | biostudies-arrayexpress
Project description:Beneficial bacteria with antibacterial properties are an attractive alternative to chemical-based antibacterial or bactericidal agents. The aim of our study was to source such bacteria from horticultural produce and environments and to explore the mechanisms of their antimicrobial properties. Four strains of Pseudomonas fluorescens were isolated that possessed antibacterial activity against the pathogen Listeria monocytogenes.
2023-03-10 | PXD019965 | Pride
Project description:Several Toll-like receptors are activated by Listeria monocytogenes infection, resulting in the activation of MyD88 dependent signaling pathway. However, the negative role of MyD88 in gene expresson is unclear. To address this, we performed microarray analysis of mRNAs from WT or MyD88-/- peritoneal macrophages infected with Listeria monocytogenes.
2014-06-29 | GSE52115 | GEO
Project description:Transcriptomic analysis of Listeria monocytogenes strains linked to fresh produce outbreaks in response to commercial sanitizers
| PRJNA1309701 | ENA
Project description:Several fresh produce outbreaks have been associated with Listeria monocytogenes contamination during postharvest. Sanitation practices are among the most critical measures for controlling and minimizing bacterial growth. The aim of this research was to evaluate the transcriptomic response of a cocktail of four L. monocytogenes strains associated with the 2011 cantaloupe and 2014 caramel apple listeriosis outbreaks. Differentially expressed genes (DEGs) were evaluated after exposure to sublethal concentrations of four sanitizer treatments including chlorine (Cl) and peracetic acid (PAA) at 5 ppm, lactic acid 0.2% (LA), and 50% silver dihydrogen citrate (SDC) during 70 s exposure via RNA-sequencing (n=4). SDC exposure resulted in the greatest number of DEGs (total: 127; up: 57, down: 70), followed by LA (total: 32; up: 8, down: 24), Cl (total: 16; up: 8, down: 8), and PAA (total: 1; up: 1). Non-coding RNAs including small, long, transfer and Listeria-specific regulatory RNAs (Rli) ranked among the most significantly DEGs across treatments. From those, one unique gene lmos88, associated with S-Adenosyl Methionine (SAM) synthesis, was significantly upregulated across all sanitizers, suggesting a fundamental role on L. monocytogenes response. Among Rlis- rli30, rli47 and rli55 were upregulated in response to Cl and SDC treatments, respectively. Both SDC and LA had significant overlap in gene response. Only Cl treatment caused significant downregulated pathways including tRNA charging (Q=0.00263), aminoacyl-tRNA biosynthesis (Q=0.00328), and other related metabolic clusters (Q=0.00355). Upregulated gene ontology (GO) terms were observed only after SDC application and were related to membrane-associated functions (Q=0.00034). These findings underscore how commercially available sanitizers affect L. monocytogenes transcriptomic profile, and the key role non-coding RNA genes play on response to sanitizers.
2026-05-21 | GSE306222 | GEO
Project description:DNA damage response kinase ATM regulates the genetic program of lymphocytes with phsiologically induced DNA DSBs. In bone marrow-derived macrophages, related kinase DNAPKcs is also responsible for activating DNA damage responses after infection with Listeria monocytogenes. Here we show that both ATM and DNA-PKcs regulate the genetic program of Listeria monocytogenes-infected macrophages.
2015-07-03 | GSE70467 | GEO
Project description:Listeria monocytogenes is a human, food-borne pathogen. Genomic comparisons between L. monocytogenes and Listeria innocua, a closely related non-pathogenic species, were pivotal in the identification of protein coding genes essential for virulence. However, no comprehensive comparison has focused on the non-coding genome. We used strand-specific cDNA sequencing to produce genome-wide transcription start site (TSS) maps for both organisms, and developed a publicly available integrative browser to visualize and analyze both transcriptomes in different growth conditions and genetic backgrounds. Our data revealed conservation across most transcripts, but significant divergence between the species in a subset of non-coding RNAs. In L. monocytogenes we identified 113 sRNAs and 70 asRNAs, significantly increasing the repertoire of non coding RNAs in this species. Remarkably, we identified a class of long antisense transcripts (lasRNAs) that overlap one gene while also serving as the 5’ UTR of the adjacent divergent gene. Experimental evidence suggests that lasRNAs transcription inhibits expression of one operon while activating the expression of another. Such lasRNA/operon structure, termed "excludon", might represent a novel form of regulation in bacteria.
2012-05-01 | GSE36060 | GEO