Project description:After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hundreds of previously unknown heteromeric complexes. Lab Heads: Andrew Emili; andrew.emili@utoronto.ca ;Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada Mohan Babu; mohan.babu@uregina.ca ;Research and Innovation Centre, University of Regina, SK S4S OA2, Canada
Project description:This SuperSeries is composed of the following subset Series: GSE31815: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Glucose at MID-log growth phase GSE31816: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + GLucose at transition-phase of growth (TS) GSE31817: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase GSE31818: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS) Refer to individual Series
Project description:Three Microarray comparisons have been preformed in this study. 1- Transcriptome comparison of the Streptococcus pneumoniae D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) to M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372597 and GSM2372598). 2- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔagaR to D39 wild type grown in M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372599 and GSM2372600). 3- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) (GSM2290636 and GSM2290637).
Project description:Streptococcus pneumoniae is a Gram-positive commensal of the nasopharynx and highly virulent opportunistic pathogen in the respiratory tract. Despite the availability of multiple vaccines, S. pneumoniae remains a significant health burden especially in aged adults resulting in higher rates of serious disease progression, hospitalization, and death following infection. Polymorphonuclear leukocytes (PMNs) are among the first responders in the lung following S. pneumoniae infection. However, studies have indicated that PMNs of human and murine origin are dysfunctional in aged individuals. To identify mechanisms underlying this dysfunction and if it displays characteristics of immunosenescence previously observed in lymphocytes, we conducted RNA sequencing on PMNs in the lungs of young and old mice at 12 and 24 hours post pulmonary infection with S. pneumoniae. Our analysis indicated significant transcriptomic differences between the young and aged cohorts associated with decreased expression of many genes important for effector function including significant metabolic changes. These metabolic changes indicated that PMNS in the lungs of aged mice fail to upregulate glycolysis, which is necessary for anti-pneumococcal activity. Analysis of the transcription factors that may be regulating these differential transcriptomic changes to pneumococcal infection indicated differential regulation by E2f2 in aged mice, which appears to negatively regulate PMN differentiation. Importantly, analysis of PMNs in aged mice display context dependent senescent-like characteristics. Together this would suggest that in aged hosts PMNs display altered maturation and can adopt a senescent-like phenotype in the periphery, which contributes to defective gene expression changes required for normal anti-pneumococcal effector functions.
Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to strain 4496.
Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to strain 4559.
Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to strain 5448.
Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to strain HKU419.
