Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe organ damage and metabolic disorders in fish. The organ damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe intestinal damage and metabolic disorders in fish. The intestinal damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
Project description:Edwardsiella ictaluri, a significant pathogen in aquaculture, can lead to severe organ damage and metabolic disorders in fish. The organ damage of hybrid grouper caused by Edwardsiella ictaluri and the alleviating effect of oligochitosan feed were verified through 16s.
Project description:We applied Solexa sequencing technology to identify Singapore grouper iridovirus (SGIV) encoded microRNAs during its infection. A small RNA library arising from SGIV infected grouper cells (GP) was constructed and sequenced. We recovered 6,802,977 usable reads, of which 34,400 reads represented the small RNA sequences encoded by SGIV. Among them, 16 novel SGIV encoded miRNAs were identified by a computational pipeline. Generally, these 16 miRNAs are dispersed throughout the SGIV genome, while three of them are located within open reading frame 057L (ORF057L) region. Meanwhile, We identified 138 conserved microRNA genes between grouper fish and zebrafish.
2011-01-29 | GSE26909 | GEO
Project description:Transcriptome sequencing of distal intestinal tissue of hybrid grouper
Project description:Fishmeal replacement with plant protein is an important strategy for sustainable aquaculture, although high soybean meal inclusion may impair growth and metabolism in carnivorous fish. This study investigated the hepatic proteomic responses of juvenile hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) fed diets containing 30% or 40% soybean meal, with or without 3% citric acid supplementation, compared with a fishmeal control diet. Liver proteins were extracted and analyzed using label-free LC-MS/MS on a Thermo Scientific Q Exactive Plus mass spectrometer. A total of 1,544 proteins were identified, and differential protein abundance, Gene Ontology enrichment, KEGG pathway analysis, and protein-protein interaction network analyses were performed to investigate metabolic adaptation associated with dietary treatments. The dataset provides molecular insights into hepatic responses to soybean meal replacement and the effect of citric acid supplementation on nutrient utilization and energy metabolism in hybrid grouper.
Project description:Grouper is an important commercial maricultural fish, which suffer viral nervous necrosis (VNN) disease at the larval and juvenile stages, but the changes of transcriptomics and proteomics during viral infection remain unknown. In this study, we applied RNA-seq and label-free mass spectrum for the first time to depict the map of transcriptomics and proteomics in non-infected, susceptible-infected and tolerate-infected grouper in larval stage. Further analyses showed that the transcriptome and proteome change dramatically among 3 distinct groups, indicating that different immune response for infected and perststent grouper in larval stage. These valuable transcriptomics and proteomics datasets enable the investigation of molecular mechanism in nervous necrosis (VNN) virus infection, thus helps the further development of molecular breeding and marine fishery
2019-05-15 | PXD012763 | Pride
Project description:Intestinal microbiota of Hybrid grouper
| PRJNA819377 | ENA
Project description:Sequencing the mitochondrial genome of the hybrid grouper
