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Transcriptome of endometrium in response to conceptus proteins in dairy cattle [ENDO]

ABSTRACT: Transcriptome of endometrium in response to conceptus proteins in dairy cattle [ENDO]

PROVIDER: PRJNA1010688 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25792300_1.fastq.gz	Fastqsanger.gz
	SRR25792300_2.fastq.gz	Fastqsanger.gz
	SRR25792301_1.fastq.gz	Fastqsanger.gz
	SRR25792301_2.fastq.gz	Fastqsanger.gz
	SRR25792302_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptome of endometrium in response to conceptus proteins in dairy cattle [ENDO]

Project description:This study reproduced in vivo aspects of the temporal signaling of interferon tau (IFNT) and pregnancy associated glycoproteins (PAG) during bovine early pregnancy. The objective of this study was to analyze the short and long-term effects of IFNT, and the effect of PAG on the transcriptome of endometrium.

2024-05-15 | GSE241884 | GEO

Unraveling the elongating bovine conceptus microenvironment: Identification of gene transcripts and proteins along the conceptus-maternal interface in cattle

Project description:The bovine conceptus elongates near Day 16 of development and releases interferon-tau (IFNT), disrupting the endometrial luteolytic mechanism to sustain luteal P4 and pregnancy. Conceptus factors other than IFNT modify local endometrial activities in support of pregnancy; however, the microenvironment is largely uncharacterized. We utilized a bovine conceptus-endometrial culture system to elucidate the microenvironment in the form of RNA and protein. Estrus synchronized heifers remained cyclic (13) or were inseminated (9) to produce Day 16 cyclic endometrium and elongating conceptuses, respectively. Conceptus sections and cyclic endometrium were then used to generate tissue cultures in 1 mL of medium: (1) no tissue (Control Med; n=7), (2) mono-cultured conceptus (Conceptus; n=9), (3) mono-cultured endometrium (Endo; n=13), or (4) Endo-Conceptus co-culture (n=15). After 12 h, tissue RNA was sequenced (RNA-Seq) and media underwent proteomic analysis (LC-MS/MS). Compared to Conceptus and Endo, co-cultured conceptus and endometrial tissue contained 3,400 and 4,575 differentially expressed genes (DEG), respectively (P ≤ 0.01). Upregulated endometrial DEG, independent of IFNT, were associated with transcription whereas upregulated conceptus DEG were associated with protein homeostasis and metabolism (P ≤ 0.001).

2024-06-30 | GSE256172 | GEO

Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

Project description:Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

| PRJNA1010686 | ENA

A bovine 3D endometrium-on-a-chip reveals the role of conceptus-derived factors CAPG and PDI in conceptus-endometrial communication

Project description:Early embryo loss affects all mammalian species, including humans and agriculturally important food-producing mammals such as cattle. The developing conceptus (embryo and extra-embryonic membranes) secretes factors which modify the endometrium and can be critical for early pregnancy processes such maternal recognition of pregnancy (MRP) and enhancing uterine receptivity to implantation. For example, a competent bovine conceptus secretes IFNT to initiate MRP. The bovine conceptus also secretes other proteins at the time of MRP, including CAPG and PDI, which are highly conserved among placental mammals. We have previously shown that these proteins act upon the endometrium to modulate receptivity, embryo development, and implantation in species with different implantation strategies (humans and cattle). We hypothesise that developing a novel 3D bovine endometrium on a chip system will enhance our understanding of the role of conceptus-derived factors in altering the endometrium and/or ULF secretion. Here we have developed a 3D bovine endometrium on a chip system, comprising both stromal and epithelial cell culture combined with culture medium flow better mimics the in vivo endometrium and exposure to conceptus-derived factors than conventional 2D endometrial cell culture. We have demonstrated that the conceptus-derived proteins CAPG and PDI modulate the endometrial transcriptome and secretory response to promote pathways associated with early pregnancy and alter ULF composition. This work highlights the critical need for more robust and in vivo-like culture systems to study endometrial-conceptus interactions in vitro to further investigate the role of conceptus derived factors for pregnancy success.

2025-05-19 | PXD059725 | Pride

A bovine 3D endometrium-on-a-chip revelas the role fo conceptus-derived factors CAPG and PDI in conceptus-endometrium communication

2025-05-19 | GSE287563 | GEO

Bovine endometrium drives and responds to divergence of in vitro produced conceptus biochemistry

Project description:In vitro produced (IVP) bovine embryos are used to enhance herd genetics and support advanced reproductive technologies, however, less than 40% survive the first month of gestation after transfer to the surrogate. While the biochemical consequences of in vitro culture on the embryo before transfer have been well documented, less is known after transfer when the embryo interacts with the endometrium and pregnancy failure occurs. To investigate this, we utilized a Day 16 trophectoderm-endometrial mono-culture and co-culture system, integrating transcriptomic and proteomic analyses, to compare crosstalk between the IVP or in vivo derived (IVD) bovine conceptus and endometrium. We found that the IVP conceptus transcriptome (differentially expressed genes; DEG), diverged from the IVD conceptus but only after co-culture with endometrium (377 DEG; FDR < 0.05). Further, of the IVP conceptus DEG, 81 genes (21%) were associated with abnormal embryonic or fetal development. Significant biological processes associated with abundant endometrial DEG induced by IVP conceptuses were related to GTPase activity and the inflammatory response (FDR < 0.01), the latter of which may be associated with increased MHC class II expression, which was specific to IVP conceptuses. Proteomic analysis of the culture media identified 1,031 and 604 differentially abundant proteins (FDR < 0.05) associated with the IVD and IVP conceptus-endometrial co-cultures, respectively, compared to endometrium alone, indicating the proteomic environment surrounding the IVP conceptus may be suboptimal. Collectively, compared to the IVD conceptus, it appears the endometrium drives and responds to divergence of IVP conceptus biochemistry, likely contributing to pregnancy failure.

2025-07-31 | GSE295732 | GEO

Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium

Project description:A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients, prior to or during the implantation window. We investigated this problem by using RNA-seq to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at d18 (pre-implantation) and d34 (post-implantation) of gestation. In addition, endometrium was profiled in order to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality prior to or during the implantation window. At d18, the effects on the transcriptome associated with SCNT were massive, involving more than 5,000 differentially expressed genes (DEGs). Among them are 121 genes that have embryonic lethal phenotypes in mice, cause defects in trophoblast and placental development, and/or affect conceptus survival in mice. In endometria at d18, <0.4% of expressed genes were affected by the presence of a cloned conceptus, whereas at d34, ~36% and <0.7% of genes were differentially expressed in intercaruncular and caruncular tissues, respectively. Functional analysis of DEGs in placental and endometrial tissues suggests a major disruption of signaling between the cloned conceptus and the endometrium, particularly the intercaruncular tissue. Our results support a bottleneck model for cloned conceptus survival during the peri-implantation period determined by gene expression levels in extra-embryonic tissues and the endometrial response to altered signaling from clones.

2016-11-26 | GSE74152 | GEO

Transcriptome of blood leukocytes in response to conceptus proteins in dairy cattle [PBLs]

Project description:Transcriptome of blood leukocytes in response to conceptus proteins in dairy cattle [PBLs]

| PRJNA1010690 | ENA

Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-05-15 | GSE241886 | GEO

Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome: effect of conceptus origin and sex

Project description:This study aimed to identify bovine conceptus-induced modifications to the endometrial transcriptome both dependent and independent of interferon tau (IFNT), dependent on conceptus origin [in vitro fertilization (IVF) or artifical insemination (AI) derived blastocysts] and dependent on conceptus sex. Major findings include identification of genes differentially expressed in endometrium in response to the conceptus but independent of IFNT and genes differentially expressed in endometrium in response to AI vs. IVF and male vs. female conceptuses.

2018-08-17 | GSE118672 | GEO

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