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Transcriptome of blood leukocytes in response to conceptus proteins in dairy cattle [PBLs]

ABSTRACT: Transcriptome of blood leukocytes in response to conceptus proteins in dairy cattle [PBLs]

PROVIDER: PRJNA1010690 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25792311_1.fastq.gz	Fastqsanger.gz
	SRR25792311_2.fastq.gz	Fastqsanger.gz
	SRR25792312_1.fastq.gz	Fastqsanger.gz
	SRR25792312_2.fastq.gz	Fastqsanger.gz
	SRR25792313_1.fastq.gz	Fastqsanger.gz

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Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

Project description:Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

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Transcriptome of blood leukocytes in response to conceptus proteins in dairy cattle [PBLs]

Project description:This study reproduced in vivo aspects of the temporal signaling of interferon tau (IFNT) and pregnancy associated glycoproteins (PAG) during bovine early pregnancy. The objective of this study was to analyze the short and long-term effects of IFNT, and the effect of PAG on the transcriptome of blood leukocytes.

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Transcriptome of endometrium and blood leukocytes in response to conceptus proteins in dairy cattle

Project description:This SuperSeries is composed of the SubSeries listed below.

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Dairy Cattle Manure

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A bovine 3D endometrium-on-a-chip reveals the role of conceptus-derived factors CAPG and PDI in conceptus-endometrial communication

Project description:Early embryo loss affects all mammalian species, including humans and agriculturally important food-producing mammals such as cattle. The developing conceptus (embryo and extra-embryonic membranes) secretes factors which modify the endometrium and can be critical for early pregnancy processes such maternal recognition of pregnancy (MRP) and enhancing uterine receptivity to implantation. For example, a competent bovine conceptus secretes IFNT to initiate MRP. The bovine conceptus also secretes other proteins at the time of MRP, including CAPG and PDI, which are highly conserved among placental mammals. We have previously shown that these proteins act upon the endometrium to modulate receptivity, embryo development, and implantation in species with different implantation strategies (humans and cattle). We hypothesise that developing a novel 3D bovine endometrium on a chip system will enhance our understanding of the role of conceptus-derived factors in altering the endometrium and/or ULF secretion. Here we have developed a 3D bovine endometrium on a chip system, comprising both stromal and epithelial cell culture combined with culture medium flow better mimics the in vivo endometrium and exposure to conceptus-derived factors than conventional 2D endometrial cell culture. We have demonstrated that the conceptus-derived proteins CAPG and PDI modulate the endometrial transcriptome and secretory response to promote pathways associated with early pregnancy and alter ULF composition. This work highlights the critical need for more robust and in vivo-like culture systems to study endometrial-conceptus interactions in vitro to further investigate the role of conceptus derived factors for pregnancy success.

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