Project description:Microarray profiling single and repeated immobilization stress responses in gene expression in rat adrenal medulla Keywords: Stress responses
Project description:Microarray profiling single and repeated immobilization stress responses in gene expression in rat adrenal medulla Experiment Overall Design: Immobilization stress (IMO) was performed with 2-hour once and 2-hour daily for 6 days repeated. Control groups were not exposed to stress (absolute controls). Following the last IMO, rats were euthanized by decapitation and both adrenal medulla tissues were dissected from the animal. Total RNA were isolated and the array experiments were done by Affymetrix. Data analysis will be performed by GeneTraffic program.
Project description:Stress is a major risk factor for many cardiovascular and neuropsyhological disorders, and involved changes in gene expression Determine target genes affected by single or repeated immboilization stress. Stress triggers alterations in gene expresssion of many proteins, especially transcription factors. The target genes of repeated stress partially overlap, but differ from those altered by single stress. Sprague Dawley male rats were exposed to single 2 hr immobilization stress or 2 hrs immbolization stress each for six consecultive days. Controls for single stress were untreated, and controls for repeated stress were handled for six days. Keywords: dose response
Project description:Stress is a major risk factor for many cardiovascular and neuropsyhological disorders, and involved changes in gene expression; Determine target genes affected by single or repeated immboilization stress. Stress triggers alterations in gene expresssion of many proteins, especially transcription factors. The target genes of repeated stress partially overlap, but differ from those altered by single stress. Sprague Dawley male rats were exposed to single 2 hr immobilization stress or 2 hrs immbolization stress each for six consecultive days. Controls for single stress were untreated, and controls for repeated stress were handled for six days.
Project description:Zonal Gene Expression and Gene Regulatory Network Responses in Centrilobular and Periportal Hepatocytes Following Repeated Exposure to TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin)
Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes
