Project description:Escherichia coli is the predominant agent causing both different disease categories of complicated urinary tract infections (UTIs) and asymptomatic bacteriuria (ASB) cases. This study employed microarray analysis to identify the differences and similarities in the expression of genes among the Uropathogenic Escherichia coli (UPEC) and two ASB E.coli isolates that represent different end continuums of its disease severities.
2026-07-31 | GSE300748 | GEO
Project description:Capsule rearrangement in multi-drug resistant Escherichia coli
| PRJNA1330052 | ENA
Project description:Compensatory evolution of multi-drug resistant Escherichia coli
Project description:Urinary tract infections (UTI) stand as one of the most prevalent infections worldwide. Uropathogenic Escherichia coli (UPEC) is the primary cause of UTI, instigating 75% of uncomplicated and 65% of complicated UTIs. Innate immune cells play pivotal roles in eliminating invading uropathogens and represent a potential therapeutic target for UTI prevention and treatment. Previous studies in our laboratory demonstrated that selective depletion of neutrophils during experimental UTI, results in an uncontrolled infection and augmented bladder and kidney pathologies. However, the exact antimicrobial mechanisms employed by neutrophils to eliminate multi-drug resistant uropathogens, such as UPEC, and resolve UTI remain poorly understood. Our dataset provides insight of the multifaceted role of neutrophil NOX2 in UPEC elimination and regulation of inflammatory and effector functions of these innate immune cells. Our transcriptome analyses revealed a heightened pro-inflammatory profile in NOX2-deficient neutrophils compared to WT neutrophils after UPEC stimulation.
Project description:Background: This study aimed to explore potential tobramycin-resistant mutagenesis of Escherichia coli (E. coli) strains after spaceflight. Methods: A spaceflight-induced mutagenesis of multi-drug resistant E.coli strain (T1_13) on the outer space for 64 days (ST5), and a ground laboratory with the same conditions (GT5) were conducted. Both whole-genome sequencing and RNA-sequencing were performed. Results: A total of 75 SNPs and 20 InDels were found to be associated with the resistance mechanism. Compared to T1_13, 1242 genes were differentially expressed in more than 20 of 38 tobramycin-resistant E. coli isolates while not in GT5. Function annotation of these SNPs/InDels related genes and differentially expressed genes was performed. Conclusion: This study provided clues for potential tobramycin-resistant spaceflight-induced mutagenesis of E. coli.
