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ABSTRACT: Bat feces metegenomic sequencing

PROVIDER: PRJNA1015157 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR25999230_1.fastq.gz	Fastqsanger.gz
	SRR25999230_2.fastq.gz	Fastqsanger.gz
	SRR25999231_1.fastq.gz	Fastqsanger.gz
	SRR25999231_2.fastq.gz	Fastqsanger.gz
	SRR25999232_1.fastq.gz	Fastqsanger.gz

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Project description:In rodents, brown adipose tissue (BAT) contributes to whole body energy expenditure and low BAT activity is related to hepatic fat accumulation, partially attributable to the gut microbiome. Little is known of these relationships in humans. In adults (n=60), we assessed hepatic fat and cold-stimulated BAT activity utilizing magnetic resonance imaging and the gut microbiome with 16S sequencing. We transplanted gnotobiotic mice with feces from humans to assess the transferability of BAT activity and NAFLD through the microbiome. Individuals with NAFLD (n=29) had lower BAT activity than those without and BAT activity was inversely related to hepatic fat. Although the fecal microbiome was different in those with NAFLD, no differences were observed in relation to BAT activity and neither of these phenotypic traits were transmissible through fecal transplant to gnotobiotic mice. Thus, low BAT activity is associated with hepatic steatosis but this is not mediated through the gut microbiota.

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