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RNA sequencing of HDFs(Human dermal fibroblasts) with or without Sal-B and UVB treatment

ABSTRACT: RNA sequencing of HDFs(Human dermal fibroblasts) with or without Sal-B and UVB treatment

PROVIDER: PRJNA1017397 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26060223_1.fastq.gz	Fastqsanger.gz
	SRR26060223_2.fastq.gz	Fastqsanger.gz
	SRR26060224_1.fastq.gz	Fastqsanger.gz
	SRR26060224_2.fastq.gz	Fastqsanger.gz
	SRR26060225_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of HDFs(Human dermal fibroblasts) with or without Sal-B and UVB treatment

Project description:Skin aging is a complex biological process that is influenced by multiple factors, including environmental and genetic factors. Ultraviolet (UV) irradiation can cause short-term sun damage, such as erythema and edema, as well as chronic damage, which accelerates skin aging and produces skin wrinkles and skin laxity. Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble extractant from the well-known herbal medicine--Salvia Miltiorrhiza in Chinese traditional medicine. Many studies have found that Sal-B exerts powerful antioxidant and anti-inflammatory effects. Therefore, we speculate that salvianolic acid B may have potential therapeutic value in the aspect of anti-photoaging.

2024-09-14 | GSE243204 | GEO

A subset of human dermal fibroblasts overexpressing Cockayne syndrome group B protein resist UVB radiation-mediated premature senescence

Project description:Ultraviolet B (UVB) radiation is a major contributor to skin photo-ageing. Although mainly absorbed by the epidermis, UVB photons managing to penetrate the upper dermis affect human dermal fibroblasts (HDFs), leading, among others, to the accumulation of senescent cells. In vitro studies have shown that repeated exposures to subcytotoxic UVB radiation doses provoke HDFs’ premature senescence shortly after the end of the treatment period. Here, we found that repetitive exposures to non-cytotoxic UVB radiation doses after several days lead to mixed cultures, containing both senescent cells and fibroblasts resisting senescence. “Resistant” fibroblasts were more resilient to a novel intense UVB radiation stimulus. RNA-seq analysis revealed that ERCC6, encoding Cockayne syndrome group B (CSB) protein, is upregulated in resistant HDFs compared to young and senescent cells, also confirmed at the protein level. CSB was found to be a key molecule conferring protection towards UVB cytotoxicity, as siRNA-mediated CSB loss-of-expression rendered HDFs significantly more susceptible to a high UVB radiation dose. In accordance, cells from a CSB-deficient patient were found to be dramatically more photosensitive. UVB-resistant HDFs remained normal (able to undergo replicative senescence) and non-tumorigenic. Even though they formed a distinct population in-between young and senescent cells, resistant HDFs retained numerous tissue-impairing characteristics of the senescence-associated secretory phenotype, including increased matrix metalloprotease activity and promotion of epidermoid tumor xenografts in immunodeficient mice. Collectively, here we describe a novel subpopulation of HDFs showing increased resistance to UVB-mediated premature senescence, as well as undesirable traits that may negatively affect skin homeostasis.

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ChIP-seq analysis of primary human dermal fibroblasts overexpressing ANKRD1 in human dermal fibroblasts (HDFs)

Project description:ChIP-seq analysis of primary human dermal fibroblasts overexpressing ANKRD1 in human dermal fibroblasts (HDFs)

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ER71/ETV2-mediated direct reprogramming of human dermal fibroblasts (HDFs) into endothelial-like cells

Project description:Samples 1-6: HDFs transduced with ER71/ETV2 were sorted on KDR expression at day 7. The isolated KDR+ cells together with human umbilical venous endothelial cells (HUVECs) and untransduced HDFs were subjected to genomic gene expression profiling. A significant number of genes related with vessel development and angiogenesis was significantly upregulated in KDR+ cells, compared to control HDFs. These findings strongly argue that ER71/ETV2 directly reprograms human fibroblasts to functional endothelial-like cells, which could be useful for disease investigation as well as autologous cell therapy. Samples 7-12: HDFs transduced with ER71/ETV2 were sorted on KDR expression at day 7. The isolated KDR+ cells were further cultured up to day 93. The further cultured cells together with human umbilical venous endothelial cells (HUVECs) and untransduced HDFs were subjected to genomic gene expression profiling. A significant number of genes related with vessel development and angiogenesis was significantly upregulated in the further cultured cells, compared to control HDFs. These findings strongly argue that ER71/ETV2 directly reprograms human fibroblasts to functional endothelial-like cells, which could be useful for disease investigation as well as autologous cell therapy. Samples 1-6: HDFs transduced with ER71/ETV2 were sorted on KDR expression at day 7. The isolated KDR+ cells together with human umbilical venous endothelial cells (HUVECs) and untransduced HDFs were subjected to genomic gene expression profiling. Samples 7-12: HDFs transduced with ER71/ETV2 were sorted on KDR expression at day 7. The isolated KDR+ cells were further cultured up to day 93. The further cultured cells together with human umbilical venous endothelial cells (HUVECs) and untransduced HDFs were subjected to genomic gene expression profiling.

2013-09-12 | E-GEOD-50801 | biostudies-arrayexpress

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Project description:Acetylated proteome data of young human dermal fibroblasts (HDFs) [Young], old HDFs [Old], FTO-knockdown HDFs [shFTO], KAT8-overexpression HDFs [KAT8] and KAT8-overexpressed HDFs after FTO deprivation [shFTO_KAT8] were analysed by LC‒MS/MS.

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ER71/ETV2-mediated direct reprogramming of human dermal fibroblasts (HDFs) into endothelial-like cells

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Project description:To define the characteristics of human oral mucosa fibroblasts (hOFs), we analyzed the gene expression of hOFs compared with that of human dermal fibroblasts (hDFs), and that of hOF-derived induced pluripotent stem cells (hOF-iPSCs). In this dataset, we found that 5,738 probes and 5672 probes were differently expressed in hOFs and hDFs respectively, compared with those in hOF-iPSCs at more than 2-fold levels. In contrast, only 434 probes are differently expressed between hOFs and hDFs.

2016-04-21 | GSE56805 | GEO

Small RNA sequencing of small extracellular vesicles derived from senescent vs. nonsenescent human dermal fibroblasts (HDFs)

Project description:Small RNA sequencing of small extracellular vesicles derived from senescent vs. nonsenescent human dermal fibroblasts (HDFs)

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proteomic analysis of gamma-tocotienol(GT3)-treated human diploid fibroblasts (HDFs)

Project description:the proteomes of untreated and GT3-treated HDFs were compared

2018-12-06 | PXD009933 | Pride

scATAC-seq of human dermal fibroblasts reprogrammed with MGT

Project description:scATAC-seq experiments were performed with control (untreated) HDFs, MGT_siControl HDFs, and MGT_siAJSZ HDFs.

2021-09-01 | GSE183121 | GEO