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Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

ABSTRACT: Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

PROVIDER: PRJNA1018579 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26102900.fastq.gz	Fastqsanger.gz
	SRR26102901.fastq.gz	Fastqsanger.gz
	SRR26102902.fastq.gz	Fastqsanger.gz
	SRR26102903.fastq.gz	Fastqsanger.gz
	SRR26102904.fastq.gz	Fastqsanger.gz

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Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

Project description:The preoptic area of hypothalamus plays an important role in sleep homeostasis. We used snRNA-seq to identify neurons involved in homeostatic sleep response.

2024-04-01 | GSE243489 | GEO

Single cell RNA-sequencing of the preoptic area of hypothalamus from sleep deprivation and rebound sleep mice

Project description:Using single-cell transcriptomics to search for hypothalamic neurons representing and controllling sleep pressure, we identified a group of neurons expressing Gpr139 in the preoptic area (POA) selectively activated during rebound sleep (RS) after sleep deprivation (SD).

2026-04-23 | GSE218624 | GEO

RNA-Seq analysis of galaninergic neurons from ventrolateral preoptic nuleus identifies expression changes between sleep and wake

Project description:Galanin neurons at ventrolateral preoptic nuleus (VLPO) have been hypothesized to be sleep-active neurons. With the use of laser capture microdissection (LCM), we specifically isolated single galanin neurons, and our goal was to assess expression changes in VLPO galanin cells between sleeping mice and mice kept awake using gentle handling.

2020-08-27 | GSE132308 | GEO

Single cell RNA-sequencing of the preoptic area of hypothalamus from sleep deprivation and rebound sleep mice

Project description:Single cell RNA-sequencing of the preoptic area of hypothalamus from sleep deprivation and rebound sleep mice

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Identification of preoptic sleep-promoting neurons based on projection target

Project description:The preoptic area (POA) of the hypothalamus is known to be crucial for sleep generation, but the spatial intermingling of sleep- and wake-promoting neurons makes it difficult to dissect the sleep control circuit. Here we identified a population of POA sleep-promoting neurons based on their projection target. Using a lentivirus for retrograde labeling with channelrhodopsin-2 (ChR2) followed by optogenetic manipulation and recording, we found that the POA GABAergic neurons projecting to the tuberomammillary nucleus (TMN) are both sleep active and sleep promoting. Cell type- and projection-specific rabies tracing revealed the presynaptic inputs to these neurons, including an amygdala GABAergic input that promotes wakefulness. Using single-cell RNA-seq, we identified several molecular markers for these neurons, and optogenetic activation of the POA neurons labeled by these markers confirmed their sleep-promoting effects. Together, these findings define a group of sleep-promoting neurons functionally, anatomically, and genetically.

2017-05-23 | GSE79108 | GEO

Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation.

Project description: Not available

| S-EPMC11197849 | biostudies-literature

Acute (2 hr) response to rxLeptin injection in preoptic area/hypothalamus/pituitary of juvenile Xenopus laevis

Project description:Leptin binding to the leptin receptor (LepR) causes rapid signaling to the nucleus. We investigated the early (2 hr) transcriptional response to acute leptin injectio (intracerebroventricular) in the preoptic area/hypothalamus/pituitary of juvenile Xenopus laevis frogs. Frogs were given i.c.v. injections of 0.6% saline or recombinant X. laevis leptin (rxLeptin; 20 ng/g BW) and 2 hrs later killed and the preoptic area/hypothalamus/pituitary dissected.

2014-08-05 | E-GEOD-60053 | biostudies-arrayexpress

Transcription profiling of mouse brain response to sleep deprivation

Project description:Examination of the effects of increasing durations of sleep deprivation. RNA is collected from two brain regions: cortex and hypothalamus.

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Acute (2 hr) response to rxLeptin injection in preoptic area/hypothalamus/pituitary of juvenile Xenopus laevis

2014-08-05 | GSE60053 | GEO

Human serum proteomics reveal molecular signature after one night of sleep deprivation

Project description:Study Objectives: Sleep deprivation is highly prevalent and caused by conditions such as night shift work or illnesses like obstructive sleep apnea. Compromised sleep is proposed to play a role in several cardiovascular, immune related and neurodegenerative disorders. We recently published human serum proteome changes after a simulated night shift. This study aimed to further explore changes in the human blood serum after 6h of sleep deprivation at night by proteomics and systems biological databases. Methods: Human blood serum samples from 8 self-declared healthy females were analyzed using mass spectrometry and high-pressure liquid chromatography. Each subject was their own control, and two samples were taken from each subject, the first one after 6h of sleep at night and the second one after 6h of sleep deprivation the following night. Biological databases and bioinformatic software were used for systems biological analyzes and comparative analysis with other published sleep-related datasets. Results: Of 494 proteins, 66 were found to be differentially expressed after 6h of sleep deprivation at night. Functional enrichment analysis revealed associations of these proteins with several biological functions related to the regulation of cellular processes like protein- and ion-binding connected to platelet degranulation and blood coagulation, as well as associations with different curated gene sets. Conclusions: This study presents serum proteomic changes after 6h of sleep deprivation, supports previous findings that only 6h of sleep deprivation affects several biological processes and revealed a molecular signature of protein changes related to pathological conditions like altered coagulation and platelet function, impaired lipid and immune function and cancer. Keywords: Human blood serum, proteomics, sleep deprivation, cellular stress, functional enrichment analysis

2024-06-27 | PXD045729 | Pride

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