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ABSTRACT: Nootkatone Rhipicephalus microplus

PROVIDER: PRJNA1019674 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26151806_1.fastq.gz	Fastqsanger.gz
	SRR26151806_2.fastq.gz	Fastqsanger.gz
	SRR26139107_1.fastq.gz	Fastqsanger.gz
	SRR26139107_2.fastq.gz	Fastqsanger.gz
	SRR26156011_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

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Project description:Transcriptional profiling of midgut tissues isolated from Rhipicephalus microplus and Rhipicephalus decoloratus females at day 20 post infestation. This enabled the identification of transcripts that are species-specific or shared between the two tick species tested. A direct comparison (Balanced block) was performed: Midgut tissues collected on day 20 (post infestation) from R. microplus was compared to that of R. decoloratus. Biological replicates: 3 ; Technical replicates: 1.

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Project description:Transcriptional profiling of whole nymphs and larvae from Rhipicephalus microplus at day 4 and 7 post infestation, respectively. This enabled the identification of transcripts that are stage-specific or shared among the stages tested.

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Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

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Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus.

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Project description:Transcriptional profiling of lymph nodes of three cattle breeds (Bonsmara, Brahman, Holstein-Friesian) in response to the cattle tick (Rhipicephalus microplus) larvae and adult infestation. Emphasis is placed on firstly, comparing transcriptional responses within a cattle breed, before and after infestation, and secondly, comparing differentially expressed genes common between the breeds.

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