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ABSTRACT: Rhipicephalus microplus Nootkatone adult

PROVIDER: PRJNA1039500 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26799534_1.fastq.gz	Fastqsanger.gz
	SRR26799534_2.fastq.gz	Fastqsanger.gz
	SRR26799248_1.fastq.gz	Fastqsanger.gz
	SRR26799248_2.fastq.gz	Fastqsanger.gz
	SRR26798553_1.fastq.gz	Fastqsanger.gz

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Project description:Nootkatone Rhipicephalus microplus

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Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

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Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

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Cattle lymph nodes: Control vs. Larva tick-infested; Control vs. Adult tick-infested; Larva tick-infested vs. Adult tick-infested

Project description:Transcriptional profiling of lymph nodes of three cattle breeds (Bonsmara, Brahman, Holstein-Friesian) in response to the cattle tick (Rhipicephalus microplus) larvae and adult infestation. Emphasis is placed on firstly, comparing transcriptional responses within a cattle breed, before and after infestation, and secondly, comparing differentially expressed genes common between the breeds.

2021-03-31 | GSE147918 | GEO

R. microplus expression profiles of larvae pre-attachment and feeding adult female stages in B. indicus and B. taurus

Project description:Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and B. taurus cattle Global analysis of gene expression changes in R. microplus during larval, pre-attachment and early adult stages of its life cycle feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. Among the 13 601 R. microplus transcripts from BmiGI Version 2 we identified 297 up and 17 down regulated transcripts were differentially expressed between R. microplus feeding on tick resistant cattle [Bos indicus (Brahman)] compared to R. microplus feeding on tick susceptible cattle [Bos taurus (Holstein-Friesian)]. These include genes encoding enzymes involved in primary metabolism, and genes related to stress, defence, cell wall modification, cellular signaling, receptor and cuticle. Microarrays were validated by qRT-PCR analysis of selected transcripts including the validation of three housekeeping genes. The analysis of all tick stages under survey suggested a coordinated regulation of defence proteins, proteases, and protease inhibitors to achieve successful attachment and survival of R. microplus on different host breeds particularly Bos indicus cattle.

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Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

Project description:Transcriptional profiling of midgut tissues isolated from Rhipicephalus microplus and Rhipicephalus decoloratus females at day 20 post infestation. This enabled the identification of transcripts that are species-specific or shared between the two tick species tested. A direct comparison (Balanced block) was performed: Midgut tissues collected on day 20 (post infestation) from R. microplus was compared to that of R. decoloratus. Biological replicates: 3 ; Technical replicates: 1.

2015-01-31 | E-GEOD-55826 | biostudies-arrayexpress

Transcriptome analysis of the immature lifestages in feeding Rhipicephalus microplus cattle ticks

Project description:Transcriptional profiling of whole nymphs and larvae from Rhipicephalus microplus at day 4 and 7 post infestation, respectively. This enabled the identification of transcripts that are stage-specific or shared among the stages tested.

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Gene expression profiling of adult female tissues in feeding Rhipicephalus microplus cattle ticks

Project description:Transcriptional profiling of salivary gland, midgut and ovary tissues isolated from Rhipicephalus microplus females at day 20 post infestation. This enabled the identification of transcripts that are tissue-specific or shared among the tissues tested.

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Microarray analysis of expressed genes from R. microplus larvae, adult ovary, adult female gut associated with B bovis.

Project description:Babesia bovis exposed tissues: Three tissues were looked at. 1. Adult Female Gut 2. Adult Ovary 3. Larvae. In all there are 24 measurements for feature (EST), and 4 measurements per treatment for each of the 6 treatment groups. The 6 treatment groups are: Gut infected (GI), Gut control (GC), and similarly for Ovary and Larval: OI, OC, LI, LC. There are 12 chips, each with a spot replicate. **Note: contact person: Felix D. Guerrero email: felix.guerrero@ars.usda.gov Keywords: Rhipicephalus (Boophilus) microplus, Babesia, microarrays.

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Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for Babesia life cycle in the vertebrate and invertebrate hosts.

Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

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