Molecular mechanisms for changing brain connectivity in mice and humans (blood samples)
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PROVIDER: PRJNA1022780 | ENA |
REPOSITORIES: ENA
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Project description:Molecular mechanisms for changing brain connectivity in mice and humans
| PRJNA1022776 | ENA
Project description:Molecular mechanisms for changing brain connectivity in mice and humans (brain samples)
| PRJNA1022783 | ENA
Project description:The goal of this study was to examine commonalities in the molecular basis of learning in mice and humans. In our previous work on mouse learning we suppressed activity in the anterior cingulate cortex (ACC) and hippocampus (HC) and found the stages for which each area was critical in performing a 2-choice visuospatial discrimination task. Our current study began by examining gene expression changes in mouse ACC and HC as a result of learning a new skill. Genes upregulated in both brain areas were used as candidates to examine commonalities between genes upregulated in mouse and human blood. We used microarrays to identify candidate genes and real-time PCR to compare the mouse results with two forms of human learning. One form involved training in a working memory task (network training), the other a generalized training shown to change many networks (meditation). We identified two genes that were upregulated in both mice and humans following training. We believe these genes act to regulate pathways that influence NF-κB, a factor previously found to be related to enhanced synaptic function and learning.
2023-10-31 | GSE244394 | GEO
Project description:The goal of this study was to examine commonalities in the molecular basis of learning in mice and humans. In our previous work on mouse learning we suppressed activity in the anterior cingulate cortex (ACC) and hippocampus (HC) and found the stages for which each area was critical in performing a 2-choice visuospatial discrimination task. Our current study began by examining gene expression changes in mouse ACC and HC as a result of learning a new skill. Genes upregulated in both brain areas were used as candidates to examine commonalities between genes upregulated in mouse and human blood. We used microarrays to identify candidate genes and real-time PCR to compare the mouse results with two forms of human learning. One form involved training in a working memory task (network training), the other a generalized training shown to change many networks (meditation). We identified two genes that were upregulated in both mice and humans following training. We believe these genes act to regulate pathways that influence NF-κB, a factor previously found to be related to enhanced synaptic function and learning.
2023-10-31 | GSE244393 | GEO
Project description:This SuperSeries is composed of the SubSeries listed below.
2023-10-31 | GSE244395 | GEO
Project description:The goal of this study was to examine commonalities in the molecular basis of learning in mice and humans. In previous work we have demonstrated that the anterior cingulate cortex (ACC) and hippocampus (HC) are involved in learning a two-choice visuospatial discrimination task. Here, we began by looking for candidate genes upregulated in mouse ACC and HC with learning. We then determined which of these were also upregulated in mouse blood. Finally, we used RT-PCR to compare candidate gene expression in mouse blood with that from humans following one of two forms of learning: a working memory task (network training) or meditation (a generalized training shown to change many networks). Two genes were upregulated in mice following learning: caspase recruitment domain-containing protein 6 (Card6) and inosine monophosphate dehydrogenase 2 (Impdh2). The Impdh2 gene product catalyzes the first committed step of guanine nucleotide synthesis and is tightly linked to cell proliferation. The Card6 gene product positively modulates signal transduction. In humans, Card6 was significantly upregulated, and Impdh2 trended toward upregulation with training. These genes have been shown to regulate pathways that influence nuclear factor kappa B (NF-κB), a factor previously found to be related to enhanced synaptic function and learning.
| S-EPMC10648558 | biostudies-literature
Project description:Purpose:This work aimed to identify the genetic profiles of piriform projection neurons and characterize their spatial organization within the piriform cortex. Methods: We microdissected the three layers of pirifrom cortex by laser capture (LMD) and performed RNA deep sequencing in order to identify layer-specific molecular markers, we then validated these data by using RNA in situ hybridization and immunohistochemistry.We next performed anterograde neural tracing experiments to identify piriform target regions, and retrograde neural tracing experiments to analyze how piriform projection neurons are organized within piriform cortex.We then combined the analysis of patterns of gene expression with retrograde tracing experiments to identify molecular signatures of the different subclasses of piriform projecting neurons. Results:We show that layers and sub-layers of the piriform cortex can be discriminated by gene expression patterns in adult piriform cortex. We observe that neurons projecting to distinct target areas are localized in distinct layers and express specific genes. We demonstrate that these molecular signatures of piriform projection neurons are maintained in reeler mice, in which cortical lamination is lost and neural positioning is scrambled, suggesting that piriform output connectivity strictly depends on the molecular programm, rather than a proper lamination of the cortex. Conclusion:These results provide important insights into the principles underling the piriform connectivity.
2016-07-13 | GSE70800 | GEO
Project description:Tumor microtubes (TMs) connect glioma cells to a network with considerable relevance for tumor progression and therapy resistance. The determination of TM-interconnectivity in individual tumors has been challenging and the impact on patient survival unresolved. Here, a connectivity signature from single-cell RNA-sequenced (scRNA-Seq) xenografted primary glioblastoma (GB) cells has been established using a dye uptake methodology, confirmed with recording of cellular calcium epochs and validated with clinical correlations. Astrocyte-like and mesenchymal-like GB cells have the highest connectivity signature scores in scRNA-sequenced patient-derived xenografts and patient samples. In large GB cohorts, network connectivity correlated with the mesenchymal subtype and dismal patient survival. CHI3L1 has been identified and validated as a robust molecular marker of connectivity with functional relevance. The connectivity signature allows novel insights into brain tumor biology, provides a proof-of-principle that tumor cell TM-connectivity is relevant for patients’ prognosis, and serves as a robust prognostic biomarker.
2023-11-27 | PXD044001 | Pride
Project description:Transplantation is a clinically relevant approach for brain repair, but much remains to be understood about influences of the disease environment in the host on transplant connectivity. To explore the influence of ageing and amyloid pathology in Alzheimer's disease (AD) we examined graft connectivity using monosynaptic Rabies virus tracing in APP/PS1 mice and in 16-18 month-old wild type mice. Neurons differentiated within 4 weeks and integrated well into the host visual cortex receiving input from the regions appropriate for visual cortex. Surprisingly, however, we found a prominent several-fold increase in local visual cortex input connectivity in both amyloid-loaded and aged environment. State-of-the-art deep proteome analysis using mass spectrometry provides first insights into the composition of environments promoting or not local exuberant input connectivity. These data therefore highlight the key role of the host pathology in shaping the input connectome calling for caution in extrapolating from one to another pathological condition.
2022-08-12 | PXD023407 | Pride
Project description:Geodia hentscheli connectivity and adaptation
| PRJEB85623 | ENA