Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1025316 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Mandarin fish Siniperca chuatsi (Basilewsky) (Percichthyidae), as a demersal piscivore, has very specialized feeding habits, for as soon as they start feeding the fry of this fish feed solely on fry of other fish species. In rearing conditions, mandarin fish has been found to accept live prey fish only, and refuse dead prey fish or artificial diets, very little is currently known about the molecular mechanisms of multiple genes which cover different pathways influencing the specialized food habit, such as live prey. We performed transcriptome comparisons between dead prey fish feeders and nonfeeders in mandarin fish. The determination mechanisms of specialized food habit (live prey fish) in mandarin fish could provide some instructions for research of food habit in animals, including mammals.
2013-10-21 | E-MTAB-1365 | biostudies-arrayexpress
Project description:Single-cell RNA sequencing was employed to investigate the host-virus interaction between Mandarin fish (Siniperca chuatsi) and Infectious spleen and kidney necrosis virus (ISKNV). Comparative analysis was performed on spleen samples obtained from ISKNV-infected individuals and healthy controls.
2026-02-11 | GSE318614 | GEO
Project description:To characterize the site-specific methylation landscape of the Mandarin fish ranavirus (MRV) genome, whole-genome bisulfite sequencing (WGBS) was conducted on an isolated MRV strain.
2024-04-02 | GSE262554 | GEO
Project description:The establishment of the immune microenvironment during oogenesis in teleosts plays an important role in individual survival and population continuation. However, the maternal influence on offspring immunity is poorly understood, particularly regarding the dynamic development and underlying mechanisms of the oocyte immune microenvironment. Here, we characterized the single-cell atlas of the mandarin fish ovary at the late stage IV, and identified three germ cells and five somatic cells. The distinctive features of the three germ cell types were further explored through the enrichment analysis, suggesting that the immune microenvironment of fish oocytes is formed during the mature oocyte stage. Subsequently, based on the pseudotemporal analysis of three germ cells, as well as the clustering analysis and the cell-cell communication analysis, we depicted the trajectories of oogenesis and its immune microenvironment formation in the mandarin fish ovary. Notably, we identified a high-low-high expression pattern in immune-related pathways across different development stages of oocytes. Furthermore, the spatiotemporal expression dynamics of six key markers were validated using RNA-FISH. These findings provide novel insights into oogenesis and the establishment of maternal/innate immunity in teleosts.
2026-06-12 | GSE298466 | GEO
Project description:Introduction: reproduction in fish, as in all vertebrates, is regulated by GnRH control on gonadotropic hormones (GTH) activity. However, the neuroendocrine factors that promote GnRH and GTH activity are unknown. In Nile tilapia (Oreochromis niloticus), sexual activity and the ability to reproduce depend on social rank; only dominant males and females reproduce. Here, this characteristic of dominant fish allows us to compare brain and pituitary gene expression in animals that do and do not reproduce, aiming to reveal mechanisms that regulate reproduction. Methods: an extensive transcriptome analysis was performed, combining two sets of transcriptomes: a novel whole-brain and pituitary transcriptome of established dominant males identified by behavioral assays, together with a cell-specific transcriptome of LH and FSH cells. Results: in most dominant fish, as determined behaviorally, the gonadosomatic index (GSI) was higher than in subordinate fish, and the leading upregulated pituitary genes were those coding for GTHs. In the brain, various neuropeptide genes, including oxytocin, cholecystokinin, and MCH, were upregulated; these may be related to reproductive status through effects on behavior and feeding. In a network analysis combining the two transcriptome sets, brain aromatase (cyp19a1b), which is specifically expressed in LH cells, is the most central gene with the highest number of connections. Conclusions: the close correlation between behavioral dominance and reproductive capacity in tilapia allows us to unravel novel genes that may partake in the regulation of the HPG axis, highlighting aromatase as an important factor in integrating the brain and pituitary factors that maintain a sexually active organism.
2022-04-30 | GSE169272 | GEO