Project description:Mandarin fish Siniperca chuatsi (Basilewsky) (Percichthyidae), as a demersal piscivore, has very specialized feeding habits, for as soon as they start feeding the fry of this fish feed solely on fry of other fish species. In rearing conditions, mandarin fish has been found to accept live prey fish only, and refuse dead prey fish or artificial diets, very little is currently known about the molecular mechanisms of multiple genes which cover different pathways influencing the specialized food habit, such as live prey. We performed transcriptome comparisons between dead prey fish feeders and nonfeeders in mandarin fish. The determination mechanisms of specialized food habit (live prey fish) in mandarin fish could provide some instructions for research of food habit in animals, including mammals.
Project description:To characterize the site-specific methylation landscape of the Mandarin fish ranavirus (MRV) genome, whole-genome bisulfite sequencing (WGBS) was conducted on an isolated MRV strain.
Project description:Single-cell RNA sequencing was employed to investigate the host-virus interaction between Mandarin fish (Siniperca chuatsi) and Infectious spleen and kidney necrosis virus (ISKNV). Comparative analysis was performed on spleen samples obtained from ISKNV-infected individuals and healthy controls.
Project description:To investigate the molecular mechanisms underlying hypoxia adaptation in mandarin fish (Siniperca chuatsi), bulk RNA sequencing was performed on MFF-1 cells. The study compares the transcriptomic profiles of cells cultured under normoxic conditions (21% O2) versus those subjected to hypoxic stress (3% O2) for 72 hours.
Project description:Mandarin fish (Siniperca chuatsi) has become one of the most commercially important freshwater aquaculture species in China because of its fast growth and high nutritional value. Here, the proteome of the spleen of pathogenetic and resistant mandarin fish on the 8th day after ISKNV infection were analyzed using Illumina NovaSeq 6000 and isobaric tag for relative and absolute quantitation. The spleen tissue of the control group (C), resistant group (K), and pathogenetic group (B) were collected at 8 dpc.
Project description:The establishment of the immune microenvironment during oogenesis in teleosts plays an important role in individual survival and population continuation. However, the maternal influence on offspring immunity is poorly understood, particularly regarding the dynamic development and underlying mechanisms of the oocyte immune microenvironment. Here, we characterized the single-cell atlas of the mandarin fish ovary at the late stage IV, and identified three germ cells and five somatic cells. The distinctive features of the three germ cell types were further explored through the enrichment analysis, suggesting that the immune microenvironment of fish oocytes is formed during the mature oocyte stage. Subsequently, based on the pseudotemporal analysis of three germ cells, as well as the clustering analysis and the cell-cell communication analysis, we depicted the trajectories of oogenesis and its immune microenvironment formation in the mandarin fish ovary. Notably, we identified a high-low-high expression pattern in immune-related pathways across different development stages of oocytes. Furthermore, the spatiotemporal expression dynamics of six key markers were validated using RNA-FISH. These findings provide novel insights into oogenesis and the establishment of maternal/innate immunity in teleosts.
2026-06-12 | GSE298466 | GEO
Project description:Trancriptome sequencing in mandarin fish
| PRJNA897748 | ENA
Project description:the tiver transcriptome of mandarin fish
| PRJNA1302142 | ENA
Project description:Transcriptome sequencing data of the Mandarin fish