Project description:In this study, 60 post-mortem tissue samples comprising four tissue types (dermis, epidermis, and muscle) were collected from autopsies of 20 Koreans aged 18 to 78. 250 ng of genomic DNA (gDNA) was employed to generate DNA methylation profiles utilizing the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA, USA) at Macrogen Inc. (Macrogen, Seoul, Korea).
Project description:In this study, 60 post-mortem tissue samples comprising four tissue types (kidney, liver, and lung) were collected from autopsies of 20 Koreans aged 18 to 78. 250 ng of genomic DNA (gDNA) was employed to generate DNA methylation profiles utilizing the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA, USA) at Macrogen Inc. (Macrogen, Seoul, Korea).
Project description:In this study, 60 post-mortem tissue samples comprising three tissue types (blood, brain, and heart) were collected from autopsies of 20 Koreans aged 18 to 78. 250 ng of genomic DNA (gDNA) was employed to generate DNA methylation profiles utilizing the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA, USA) at Macrogen Inc. (Macrogen, Seoul, Korea).
Project description:Previous studies indicate that autism has a strong genetic component; however, the underlying genetic mechanisms remain unclear. Recently, it was reported that genetic heritability is lower than previously estimated, and that environmental factors have a greater influence on the development of autism spectrum disorder (ASD). Epigenetic processes such as DNA methylation are considered to be at the interface of genetic and environmental factors. Additionally, two well-characterized epigenetic processes, parental imprinting and X chromosome inactivation, are involved in several conditions that mimic autism spectrum disorders, such as Rett and Fragile-X syndromes, suggesting that epigenetic processes may play an important role in the pathophysiology of autism. We investigated genome-wide DNA methylation profiles in post-mortem brain tissue from individuals with autism using the Infinium HumanMethylation450 BeadChip (Illumina), which includes CpG sites of intragenic and intergenic regions.