Project description:Gut microbial profiling of uterine fibroids (UFs) patients comparing control subjects. The gut microbiota was examined by 16S rRNA quantitative arrays and bioinformatics analysis. The goal was to reveal alterations in the gut microbiome of uterine fibroids patients.
Project description:The gut microbiota plays a critical role in host energy metabolism and immune regulation, yet the temporal dynamics of microbial community responses to acute physiological stressors remain poorly characterized. While exercise is recognized as a modulator of gut microbial composition, the immediate post-exercise shifts in microbial community structure across different genetic backgrounds have not been systematically examined. Here, we present a longitudinal 16S rRNA gene sequencing dataset profiling the temporal dynamics of gut microbiota following acute high-intensity exercise in two widely used laboratory mouse strains. Age-matched male BALB/c and C57BL/6J mice were subjected to a single bout of acute exercise. The colonic content samples were collected to capture early temporal responsesat. Total genomic DNA was extracted from colonic contents and the 16S rRNA V4 region was amplified and sequenced on the NovaSeq6000 platform. This dataset enables identification of strain-specific and conserved exercise-responsive microbial taxa, and supports functional predictions related to energy metabolism and intestinal homeostasis.
Project description:The impact of mono-chronic S. stercoralis infection on the gut microbiome and microbial activities in infected participants was explored. The 16S rRNA gene sequencing of a longitudinal study with 2 sets of human fecal was investigated. Set A, 42 samples were matched, and divided equally into positive (Pos) and negative (Neg) for S. stercoralis diagnoses. Set B, 20 samples of the same participant in before (Ss+PreT) and after (Ss+PostT) treatment was subjected for 16S rRNA sequences and LC-MS/MS to explore the effect of anti-helminthic treatment on microbiome proteomes.
Project description:The study investigated the impact of environment on the composition of the gut microbiota and mucosal immune development and function at gut surfaces in early and adult life. Piglets of similar genotype were reared in indoor and outdoor environments and in an experimental isolator facility. Mucosa-adherent microbial diversity in the pig ileum was characterized by sequence analysis of 16S rRNA gene libraries. Host-specific gene responses in gut ileal tissues to differences in microbial composition were investigated using Affymetrix microarray technology and Real-time PCR.
Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:Interventions: Case (colorectal cancer) group:a newly diagnosed colorectal cancer( CRC ) by colonoscopy and pathology;Control group:Clinically healthy volunteers with no symptoms or history of intestinal disease(e.g. colonic adenomatous polyps, CRC or inflammatory bowel disease)
Primary outcome(s): composition of gut microbiota;intestinal microbial phytase activity;16s rRNA metagenomic sequencing;diet surveys;phytic acid intake
Study Design: Case-Control study
Project description:A phylogenetic microarray targeting 66 families described in the human gut microbiota has been developped aud used to monitor the gut microbiota's structure and diversity. The microarray format provided by Agilent and used in this study is 8x15K. A study with a total of 4 chips was realized. Arrays 1 and 2: Hybridization with 100ng of labelled 16S rRNA gene amplicons from a mock community sample and 250ng of labelled 16S rRNA gene amplicons from 1 faecal sample. Each Agilent-030618 array probe (4441) was synthetized in three replicates. Arrays 3 and 4: Hybridization with 250ng of labelled 16S rRNA gene amplicons from 2 faecal samples. Each Agilent-40558 array probe (4441) was synthetized in three replicates.
Project description:Investigation of microbial community composition in mouse models using an intestinal epithial-specific and inducible VilCreERT2-mediated conditional knockout of Jup under basal conditions and in acute dextran-sodium sulfate (DSS)-induced colitis We investigated the gut microbiome composition in a dextran sulfate sodium (DSS) colitis model using Jupfl/fl and iVilCreERT2Jupfl/fl mice. Fecal samples were collected after DSS treatment, and 16S rRNA sequencing was employed to analyze microbial communities. Our findings revealed no significant differences in microbial profiles between Jupfl/fl and iVilCreERT2Jupfl/fl under DSS treatment.
Project description:The present study was conducted in the frame of the EU-funded Graphene Flagship project. We previously evaluated the impact of graphene oxide (GO) on the gut microbiome in adult zebrafish by performing 16S rRNA gene sequencing in wild-type versus AhR-deficient zebrafish. Here, we performed single-cell RNA-sequencing (10x Genomics) on whole (dissociated) germ-free (GF) zebrafish embryos exposed at 5 dpf to GO plus the microbial metabolite butyrate to gain insight into the impact on specific cell populations in GF zebrafish.
