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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA sequencing of germ-free zebrafish embryos

ABSTRACT: The present study was conducted in the frame of the EU-funded Graphene Flagship project. We previously evaluated the impact of graphene oxide (GO) on the gut microbiome in adult zebrafish by performing 16S rRNA gene sequencing in wild-type versus AhR-deficient zebrafish. Here, we performed single-cell RNA-sequencing (10x Genomics) on whole (dissociated) germ-free (GF) zebrafish embryos exposed at 5 dpf to GO plus the microbial metabolite butyrate to gain insight into the impact on specific cell populations in GF zebrafish.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Danio rerio

SUBMITTER: Bengt Fadeel

PROVIDER: E-MTAB-11984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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