Project description:Transcriptome profiling analysis of the Hansenula polymorpha MET4 gene deletion strain have been carried out to obtain comprehensive information on the HpMet4p-mediated regulatory networks in association with the cadmium (Cd) detoxification and sulfur regulation in H. polymorpha.
Project description:Transcriptome profiling analysis of the Hansenula polymorpha MET4 gene deletion strain have been carried out to obtain comprehensive information on the HpMet4p-mediated regulatory networks in association with the cadmium (Cd) detoxification and sulfur regulation in H. polymorpha. Total RNA samples were collected from H. polymorpha wild type, and HpMET4 deletion strain, under sulfur starvation or Cd (0.6 mM) stress conditions. The differential fluorescence intensities of each RNA sample were measured after labeling with Cy3 or Cy5. For all analyses, we performed dye swapping experiments to avoid dye bias. Thus, four intensity values were generated for each ORF and averaged for analysis.
Project description:To gain insight into the basic mechanism of Cd detoxification in the methylotrophic yeast, H. polymorpha, we analyzed temporal changes in transcriptional profiles in response to Cd exposure. We used H. polymorpha whole-genome cDNA microarrays, which contain 98% of all H. polymorpha ORFs in duplicate or triplicate. Keywords: time course, cadmium
Project description:Purpose: To identify the downstream gene sets regulated by Mpk1p in H. polymorpha under normal and caffeine-treated conditions, we compared the RNA-seq based transcriptional profiles. Methods: Total RNA was isolated according to the hot phenol extraction method and all procedure for RNA sequencing were conducted by ChunLab. More than 1.5-fold regulated genes are functionally classified using the Munich Information Center for Protein Sequences (MIPS) FunCat. Results: Transcriptome analysis of the DL1-L strain revealed that caffeine significantly increased expression of a subset of cell-wall related genes in an Mpk1p-dependent manner, but most of which were not Rlm1-target genes. Conclusion: Our results support the essential role of Mpk1p in maintaining cell wall integrity upon several stress conditions of H. polymorpha.
