Project description:To investigate the potential role of miRNA-148b-5p in the pathogenesis of kidney stones, we conducted RIP-seq of rat's kidney tissue.
Project description:To investigate the transcriptome differences in kidney stones process, we established CaOx-induced nephrolithiasis mouse model and performed RNA-sequencing.
Project description:Kidney stones are prevalent. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of cells in mouse kidneys with nephrolithiasis.
2024-12-25 | GSE221461 | GEO
Project description:Intestinal microorganisms of SD rats with kidney stones Raw sequence reads
Project description:To investigate the transcriptome differences between wild-type and Vhl mutant mice in kidney stones process, we established CaOx-induced nephrolithiasis mouse model and performed RNA-sequencing.
Project description:Formation of salivary stones, a rare pathological condition, currently lacks non-surgical treatment options. The underlying causes remain unknown, although one hypothesis suggests bacterial infections may play a role. To investigate these theories and identify proteins whose altered levels might impact salivary gland homeostasis, leading to stone formation, optimal sample processing protocols were developed. The objective was to maximise protein extraction efficiency, evaluating sonication enhancement for this purpose. To establish a universal protocol applicable to various biological materials, different sample types—saliva, salivary stones, kidney stones, salivary glands, and Raoultella ornithinolytica—were processed and analysed. Since sonication increased the number of detected proteins, the subsequent step involved assessing the feasibility of quantitative analysis. Proteins identified in saliva and salivary stones were compared, revealing seventy-four overlapping proteins primarily associated with bacterial infections and extracellular regions, aligning with previous findings from salivary stone analysis alone. It suggests the potential for selecting high-confidence biomarkers responsible for stone formation, particularly by analysing saliva from healthy donors as a control group. Furthermore, 56 proteins were detected in both salivary stones and kidney stones, suggesting the potential to identify proteins crucial for stone formation across various diseases or pathological states.
2026-03-11 | PXD069065 | Pride
Project description:The microbiome of kidney stones and urine of patients with nephrolithiasis
