Proteomic profiling of saliva, salivary stones and kidney stones reveals candidate biomarkers of pathological calcification
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ABSTRACT: Formation of salivary stones, a rare pathological condition, currently lacks non-surgical treatment options. The underlying causes remain unknown, although one hypothesis suggests bacterial infections may play a role. To investigate these theories and identify proteins whose altered levels might impact salivary gland homeostasis, leading to stone formation, optimal sample processing protocols were developed. The objective was to maximise protein extraction efficiency, evaluating sonication enhancement for this purpose. To establish a universal protocol applicable to various biological materials, different sample types—saliva, salivary stones, kidney stones, salivary glands, and Raoultella ornithinolytica—were processed and analysed. Since sonication increased the number of detected proteins, the subsequent step involved assessing the feasibility of quantitative analysis. Proteins identified in saliva and salivary stones were compared, revealing seventy-four overlapping proteins primarily associated with bacterial infections and extracellular regions, aligning with previous findings from salivary stone analysis alone. It suggests the potential for selecting high-confidence biomarkers responsible for stone formation, particularly by analysing saliva from healthy donors as a control group. Furthermore, 56 proteins were detected in both salivary stones and kidney stones, suggesting the potential to identify proteins crucial for stone formation across various diseases or pathological states.
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human) Raoultella Ornithinolytica
TISSUE(S): Salivary Gland, Saliva
SUBMITTER:
Natalia Musiał
LAB HEAD: Paulina Czaplewska
PROVIDER: PXD069065 | Pride | 2026-03-11
REPOSITORIES: Pride
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