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Mesendoderm differentiation of WT and PHD2 knockout mESCs (scRNA-seq)

ABSTRACT: mesendoderm differentiation of WT and PHD2 knockout mESCs (scRNA-seq)

PROVIDER: PRJNA1028512 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26394382_1.fastq.gz	Fastqsanger.gz
	SRR26394382_2.fastq.gz	Fastqsanger.gz
	SRR26394383_1.fastq.gz	Fastqsanger.gz
	SRR26394383_2.fastq.gz	Fastqsanger.gz
	SRR26394384_1.fastq.gz	Fastqsanger.gz

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mesendoderm differentiation of WT and PHD2 knockout mESCs (scRNA-seq)

Project description:To study the cell composition and gene expression profile in the mesendoderm differentiation of WT and PHD2 knockout, we performed scRNA-seq on the cells collected from the differentiation of WT and PHD2 knockout AB2.2 mESCs at differentiation day 4. PHD2 knockoutout mESCs were constructed by CRISPR/Cas9. The differentiation was performed using a non-lineage tendency differentiation protocol.

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mesendoderm differentiation with Hif-1a knockdown

Project description:To study the gene expression profile in the mesendoderm differerntiation of scramble control and Hif-1a knockdown, we performed RNA-seq on the total RNA samples collected from the differentiation of scramble and Hif-1a knockdown AB2.2 mESCs at differentiation day 4. Sramble and Hif-1a knockdown AB2.2 mESCs were built by infecting AB2.2 cells with lentiviruses. Each group contained three biological replicates. The expression matrix was obtained by Hisat2 followed by Stringtie.

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Project description:To study the gene expression profile in the mesendoderm differerntiation of control and Hif-1a overexpression, we performed RNA-seq on the total RNA samples collected from the differentiation of control and Hif-1a overexpression AB2.2 mESCs at differentiation day 4.In addition, to study the gene expression profile in the mesendoderm differerntiation of Hif-1a knockdown under normoxia and hypoxia, we performed RNA-seq on the total RNA samples collected from the differentiation of Hif-1a knockdown AB2.2 mESCs under normoxia or hypoxia at differentiation day 4. Cell lines were built by infecting AB2.2 cells with lentiviruses. Each group contained three biological replicates. The expression matrix was obtained by Hisat2 followed by Stringtie.

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