Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Population genomics of Poa trivialis

PROVIDER: PRJNA1034650 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26632900.fastq.gz	Fastqsanger.gz
	SRR26633000.fastq.gz	Fastqsanger.gz
	SRR26632901.fastq.gz	Fastqsanger.gz
	SRR26633001.fastq.gz	Fastqsanger.gz
	SRR26632902.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 178

Similar Datasets

Identification of preoptic sleep-promoting neurons based on projection target

Project description:The preoptic area (POA) of the hypothalamus is known to be crucial for sleep generation, but the spatial intermingling of sleep- and wake-promoting neurons makes it difficult to dissect the sleep control circuit. Here we identified a population of POA sleep-promoting neurons based on their projection target. Using a lentivirus for retrograde labeling with channelrhodopsin-2 (ChR2) followed by optogenetic manipulation and recording, we found that the POA GABAergic neurons projecting to the tuberomammillary nucleus (TMN) are both sleep active and sleep promoting. Cell type- and projection-specific rabies tracing revealed the presynaptic inputs to these neurons, including an amygdala GABAergic input that promotes wakefulness. Using single-cell RNA-seq, we identified several molecular markers for these neurons, and optogenetic activation of the POA neurons labeled by these markers confirmed their sleep-promoting effects. Together, these findings define a group of sleep-promoting neurons functionally, anatomically, and genetically.

2017-05-23 | GSE79108 | GEO

DNMT inhibition in the Neonatal Preoptic Area

Project description:PN2 male and female rat pups were treated with vehicle or zebularine. POA RNA was extracted and sequenced to deterime the effects of DNMT inhibition in POA gene expression. Males and Females were treated with either vehicle (0.1% DNMSO in saline) or Zebularine

2015-03-30 | E-GEOD-66203 | biostudies-arrayexpress

Single-cell proteomics analysis of melatonin-treated and MG132-treated in vitro post-ovulatory aged oocytes

Project description:Once ovulated, the oocyte has to be fertilized in a short time window, or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell proteomic and phosphoproteomic analysis of fresh, POA, melatonin-treated and MG132-treated POA oocytes.

2025-05-06 | PXD045614 | Pride

DNMT inhibition in the Neonatal Preoptic Area

Project description:PN2 male and female rat pups were treated with vehicle or zebularine. POA RNA was extracted and sequenced to deterime the effects of DNMT inhibition in POA gene expression.

2015-03-30 | GSE66203 | GEO

Expression profile of adult mouse POA/BF regions

Project description:The adult mammalian brain is composed of distinct regions that have specialized roles. The BF/POA regions are thought to have an important role in the regulation of sleep/wake behavior. However, genetic markers of the responsible cells for the regulation of sleep/wake behavior are largely unknown. To identify the molecular markers of the BF/POA regions, we sampled the BF/POA regions and compared gene expression in the BF/POA regions with those of other brain regions which we previously reported in the BrainStars (B*) project, in which we sampled ~50 small brain regions, including sensory centers and centers for motion, time, memory, fear, and feeding. We sampled each region every 4 hours for 24 hours, and pooled the sample sets for DNA-microarray assays. We then used informatics to identify candidates for genes with high or low expression in the BF/POA regions. We used our findings to develop an integrated database (http://poabf.brainstars.org/) for exploring genome-wide expression in the adult mouse brain including the BF/POA regions.

2018-08-17 | GSE107500 | GEO

RNASeq of cold-sensitive and cold-insensitive neurons from the pre-optic area of hypothalamus of mice

Project description:The preoptic area of the hypothalamus (POA) contains intrinsically warm and cold-sensitive neurons, which are thought to be critically involved in mammalian thermoregulation. However, the precise physiological roles and the molecular markers of the cold-sensitive POA neurons have not been determined yet. Here, we tackle this problem by performing calcium-imaging guided separation and collection of cold-sensitive and cold-insensitive dissociated neurons from the mouse POA, followed by RNASeq and differential transcriptomics of these cell populations.

2020-04-09 | GSE136396 | GEO

Anthus trivialis

Project description:Anthus trivialis

| PRJNA919852 | ENA

ihCloTriv

Project description:Closterotomus trivialis

| PRJEB53562 | ENA

Project description:Pseudomonas trivialis BS3111

| PRJEB16396 | ENA

Pseudomonas trivialis

Project description:Pseudomonas trivialis overview

| PRJNA303927 | ENA