Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1034949 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:How species genetically adapt to new environments is a central question in evolutionary biology. Here whole-genome sequencing combined with functional analysis is used to dissect how Atlantic herring, a marine fish, has adapted to the brackish Baltic Sea. Genes involved in reproduction and early development emerge as primary targets of natural selection, with key changes in a sperm-specific anion channel (LRRC8C2), a zona pellucida protein (ZPBA1), a cluster of three genes for fish transglutaminase (FTG1-3), and a copy number expansion of a fish hatching enzyme gene (HE1C). The large diameter of LRRC8C2 homomers facilitates transport of ions and osmolytes, likely preventing swelling of sperm when spawning in low salinity. Altered ZPBA1 sequence together with modified FTG1-3 enzyme activity produces a harder egg envelope that prevents egg swelling in brackish waters, while the enhanced activity of the adapted HE1C enzyme enables larvae to digest this reinforced egg envelope during hatching. Baltic Sea herring populations reproducing in brackish water are fixed or nearly fixed for variant alleles at these four unlinked loci, each carrying multiple amino-acid substitutions compared to the alleles prevalent in the Atlantic Ocean populations. The alleles at three of these loci (ZPBA1, FTG1-3, and HE1C) have been introgressed from Pacific herring populations present in the Arctic Sea. These findings reveal concrete molecular mechanisms by which a marine species has adapted to a novel, low-salinity environment.
2026-04-18 | PXD076334 | panorama
Project description:Whole-genome sequencing data of three Sebastes species
| PRJNA507287 | ENA
Project description:Seafood fraud has become a global emerging issue, threatening food security and safety. Adulteration, substitution, dilution, and incorrect labeling of seafood products are fraudulent practices that violate consumer safety. In this context, developing sensitive, robust, and high-throughput molecular tools for food and feed authentication is becoming crucial for regulatory purposes. Analytical approaches such as proteomics mass spectrometry have shown promise in detecting incorrectly labeled products. For the application of these tools, genome information is crucial, but currently, for marine species of commercial importance, such information is unavailable. However, when combining proteomic analysis with spectra library matching, commercially important fish species were successfully identified, differentiated, and quantified in pure muscle samples and mixtures, even when genome information was scarce. This study further tested the previously developed proteomic-based spectra library-based approach was further tested to differentiate 29 fish species from the North Sea in individual samples, laboratory-prepared mixtures, and commercial samples. For authenticating libraries generated from 29 fish species, fresh muscle samples from the fish samples were matched against the reference libraries. Species of the fresh fish samples were correctly authenticated using the spectra libraries generated from the 29 fish species. Furthermore, processed commercial products containing mixtures of two or three fish species were matched against these spectra libraries to test the accuracy and robustness of this method for authentication of fish species. The results indicated that the method is suitable for the authentication of fish species from highly processed samples such as fish cakes and burgers. Spectra libraries built from 29 fish species in the North Sea can efficiently tackle current and future challenges in feed and food authentication analyses when prospecting new resources in the Arctic.
2024-04-23 | MSV000094602 | MassIVE
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
| 2533916 | ecrin-mdr-crc
Project description:We set out to investigate the genetic adaptions of the known marine fungus Paradendryphiella salina CBS112865 to the degradation of brown macro-algae, expecting to find a repertoire of carbohydrate active enzymes highly specialized to the degradation of algal polysaccharides. We performed whole genome, transcriptome sequencing and shotgun proteomic analysis of the secretome of P. salina growing on three species of brown algae and under carbon starvation. The genome comparison to close terrestrial fungal relatives, revealed P. salina to have a similar, but reduced carbohydrate active enzyme (CAZyme) profile, except for the presence of three putative alginate lyase 7 genes, most likely acquired via ancient horizontal gene transfer event from a marine bacterium and a polysaccharide lyase 8 gene with similarity to ascomycete chondroitin AC lyases. The proteomic analysis revealed both PL7 and PL8 enzymes to be highly abundant in the algal fermentations together with enzymes necessary for degradation of laminarin, cellulose, lipids and peptides. Our findings indicate that the base CAZyme repertoire of saprobic and plant pathogenic ascomycetes with the necessary addition of alginate lyases provide the fungi with the enzymatic capabilities to thrive on brown algae polysaccharides and even cope with the algal defense mechanisms.
2019-11-13 | PXD012152 | Pride
Project description:The swamp eel or rice field eel (Monopterus albus) taxonomically belongs to the family Synbranchidae of the order Synbranchiformes (Neoteleostei, Teleostei, Vertebrata). It is not only an economically important freshwater fish in aquacultural production, but also an increasingly known model species for biological studies. Understanding molecular mechanisms underlying sex change is a major area of interest. The swamp eel thus offers a powerful system for studying sexual development and adaptive evolution in vertebrates.The whole genome sequencing provides valuable resources for sex control in fish production, species protection through manipulating sex reversal genes, and potentially enabling effective population control and promoting reproduction health in human. High throughput sequencing was employed for three samples,three kind s of sex gonad from swamp eel, testis,ovotestis and ovary, no replicates.
2016-07-03 | E-GEOD-43649 | biostudies-arrayexpress
Project description:Whole genome sequence data of three Actinidia species
| PRJDB13926 | ENA
Project description:Transcriptional responses to hypoxia were assessed in three species of marine fish (smoothhead sculpin [Artedius lateralis], sailfin sculpin [Nautichthys oculafasciatus] and Pacific staghorn sculpin [Leptocottus armatus]) all of which have previously been shown to share the same level of hypoxia tolerance. To determine if there is convergence in mechanisms underlying phenotypic convergence of hypoxia tolerance, each species was exposed to short-term (8 hours) and long-term (72 hours) hypoxia. Specifically, liver was sampled and mRNA was extracted from each species at normoxia, 3hr, 8hr, 24hr, 48hr and 72hr of hypoxia.
2018-10-11 | E-MTAB-6233 | biostudies-arrayexpress