Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of WT vs Wdr47 deficient primary neurons

ABSTRACT: Gene expression analysis of WT vs Wdr47 deficient primary neurons

PROVIDER: PRJNA1036545 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26700887.fastq.gz	Fastqsanger.gz
	SRR26700888.fastq.gz	Fastqsanger.gz
	SRR26700889.fastq.gz	Fastqsanger.gz
	SRR26700890.fastq.gz	Fastqsanger.gz
	SRR26700891.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Global proteome of Atg5flox:CAG-CreTmx WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg5flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

2022-11-15 | PXD031487 | Pride

Global proteome of Atg16L1flox:CAG-CreTmx WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg16L1flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

2022-11-15 | PXD031555 | Pride

ov_IRF9 vs WT

Project description:ov_IRF9 vs WT

| PRJNA1240192 | ENA

Gene expression comparison, WT and PDAP1-deficient primary B lymphocytes

Project description:Gene expression comparison, WT and PDAP1-deficient primary B lymphocytes

| PRJNA595013 | ENA

Gene expression analysis of WT vs Wdr47 deficient primary neurons

Project description:Corpus callosum (CC) is the largest interhemispheric connection that is largely formed by the axons of layer 2/3 callosal projection neurons (CPNs) through a series of tightly regulated cellular events.Defects in any of those steps may prevent the proper development of the corpus callosum resulting in a spectrum of disorders collectively referred to as corpus callosum dysgenesis (CCD). Here, we report 5 patients carrying bi-allelic variants in WDR47 presenting CCD together with microcephaly. Using a combination of in vitro and in vivo mouse models and complementation assays, we show that independently from its previously identified functions in neuronal migration and neurite extension, Wdr47 is required for survival of callosal neurons by contributing to the maintenance of mitochondrial and microtubule homeostasis. We further provide evidence that severity of the CCD phenotype is determined by the degree of the loss of function caused by the variants. Taken together, we identify WDR47 as a novel causative gene for corpus callosum abnormalities.

2024-11-06 | GSE247160 | GEO

Global proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain excitatory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:CamKIIα-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:CamKIIα-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and excitatory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031552 | Pride

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and inhibitory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031535 | Pride

RNA-seq analysis of WT and H2BE-KO primary cortical neurons

Project description:RNA-seq analysis of WT and H2BE-KO primary cortical neurons

| PRJNA1034131 | ENA

PTPN2 knockout vs WT

Project description:PTPN2 knockout vs WT

| PRJNA871237 | ENA

UPAB1 secretome analysis of hlyD1 vs hlyD2 vs Wt.

Project description:A. baumannii UPAB1 secretome analysis of hlyD1 vs Wt and hlyD2 vs Wt.

2021-08-09 | PXD024736 | Pride