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Exploring Epigenetic Changes in the Development of Gemcitabine Resistance [SW1990R_vs_SW1990_RRBS]

ABSTRACT: Exploring Epigenetic Changes in the Development of Gemcitabine Resistance [SW1990R_vs_SW1990_RRBS]

PROVIDER: PRJNA1036556 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26700306_1.fastq.gz	Fastqsanger.gz
	SRR26700306_2.fastq.gz	Fastqsanger.gz
	SRR26700307_1.fastq.gz	Fastqsanger.gz
	SRR26700307_2.fastq.gz	Fastqsanger.gz

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Exploring Epigenetic Changes in the Development of Gemcitabine Resistance [SW1990R_vs_SW1990_ATACseq]

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Exploring Epigenetic Changes in the Development of Gemcitabine Resistance [SW1990R_vs_SW1990_ATACseq]

Project description:To elucidate the epigenetic events, particularly the dynamic alterations in chromatin accessibility during the development of gemcitabine resistance in pancreatic cancer cells, both the parental SW1990 cells and the gemcitabine-resistant counterparts, SW1990R cells, were employed for mapping of the chromatin accessibility landscape. The ATAC-seq technology was used for precise detection of chromatin accessibility.

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Exploring Epigenetic Changes in the Development of Gemcitabine Resistance [SW1990R_vs_SW1990_CUTTAG]

Project description:To elucidate the epigenetic events, particularly the dynamic alterations in chromatin states during the development of gemcitabine resistance in pancreatic cancer cells, both the parental SW1990 cells and the gemcitabine-resistant counterparts, SW1990R cells, were employed for mapping of the histone modification landscape. The CUT&Tag-seq technology was used for precise detection of histone marks, including H3K4me1 (associated with enhancers), H3K27ac (related to active enhancers and promoters), H3K4me3 (linked to PolII-bound and CpG-rich promoters), H3K9me3 (associated with heterochromatin regions), and H3K27me3 (indicative of Polycomb inhibition).

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Project description:To elucidate the DNA methylation alterations during the development of gemcitabine resistance in pancreatic cancer cells, we utilized both the parental SW1990 cells and their gemcitabine-resistant derivatives, SW1990R cells, to examine DNA methylation patterns. We employed reduced representation bisulfite sequencing (RRBS) assays to uncover the specific CpG methylation profiles.

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Project description:To investigate the transcriptional alterations that occur during the development of gemcitabine resistance in pancreatic cancer cells, we established gemcitabine-resistant SW1990 cells, referred to as SW1990R. We then employed RNA-seq to explore the transcriptome changes between the gemcitabine-resistant cells and their parental counterparts.

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