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Cross-talks between metabolic and translational controls during beige adipocyte differentiation [Ribo-seq]

ABSTRACT: Cross-talks between metabolic and translational controls during beige adipocyte differentiation [Ribo-seq]

PROVIDER: PRJNA1040202 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26823286.fastq.gz	Fastqsanger.gz
	SRR26823287.fastq.gz	Fastqsanger.gz
	SRR26823288.fastq.gz	Fastqsanger.gz
	SRR26823289.fastq.gz	Fastqsanger.gz
	SRR26823290.fastq.gz	Fastqsanger.gz

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Cross-talks between metabolic and translational controls during beige adipocyte differentiation [Ribo-seq]

Project description:Whether and how regulatory events at the translation stage shape the cellular and metabolic features of thermogenic adipocytes is hardly understood. In this study, we report two hitherto unidentified cross-talk pathways between metabolic and translation regulation in beige adipocytes. By analysing temporal profiles of translation activity and protein level changes during precursor-to-beige differentiation, we found selective translational down-regulation of OXPHOS component-coding mRNAs. The down-regulation restricted to Complexes I, III, IV, and V, is coordinated with enhanced translation of TCA cycle genes, engendering distinct stoichiometry of OXPHOS and TCA cycle components and altering the related metabolic activities in mitochondria of thermogenic adipocytes. Our high-resolution description of ribosome positioning unveiled potentiated ribosome pausing at glutamate codons. The increased stalling is driven by elevated glutamate consumption that diminishes glutamate-charged tRNA during pan-adipocyte differentiation. The ribosome pauses decrease protein synthesis and mRNA stability of glutamate codon-rich genes, such as actin cytoskeleton-associated genes.

2025-03-03 | GSE247713 | GEO

Cross-talks between metabolic and translational controls during beige adipocyte differentiation

Project description:Cross-talks between metabolic and translational controls during beige adipocyte differentiation

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Cross-talks between metabolic and translational controls during beige adipocyte differentiation [RNA-seq]

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Cross-talks between metabolic and translational controls during beige adipocyte differentiation [RNA-seq]

Project description:Cross-talks between metabolic and translational controls during beige adipocyte differentiation [RNA-seq]

| PRJNA1040201 | ENA

Cross-talks between Metabolic and Translational Controls during Beige Adipocyte Differentiation

Project description:Measurement of protein levels upon beige adipocyte differentiation. Adipocyte precursor cells were isolated from inguinal white adipose tissue, and induction of beige adipocyte differentiation was performed. Non-induced and differentiated samples for 4 or 8 days were used for sample preparation. For each condition, triplicates were constructed. Peptide fragmentation was performed for 9 samples, and then an equal volume of each sample was pooled to generate Ref 1 and 2. Next, a total of 11 samples were labeled with 11 different TMT reagents. Labeled samples were pooled into one. Then, 11 fractions were acquired from the HPLC fraction system, and LS-MS/MS of each fraction was performed.

2023-11-13 | MSV000093396 | MassIVE

Molecular basis of beige adipocyte maintenance

Project description:Molecular basis of beige adipocyte maintenance

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Genome-wide binding sites of Foxp4 in beige adipocyte

Project description:We found deletion of Foxp4 in mature adipocytes would augment juvenile and mature beige adipocyte thermogenesis.In order to investage the binding site of Foxp4 in the genome of beige adipocytes, we did Foxp4 ChIP-Seq with differentiated SVF cells derived from ingunal adipose tissues.

2022-04-06 | GSE199559 | GEO

M2 macrophages independently promote beige adipogenesis via blocking adipocyte Ets1

Project description:Beige adipocytes accumulates mitochondria and alleviates metabolic disorder via activating energy expenditure. Whether the opposing process of mitochondrial biogenesis and clearance are integrated regulated in beige adipocytes is beyond known. Here we show that DNA binding protein Ets1 suppresses beige adipocyte formation via bidirectional regulation of mitochondrial biogenesis and clearance. The expression level of Ets1 was down-regulated in browning adipocytes, and up-regulated in the subcutaneous fat of obese mice. Adipocyte Ets1 heterozygous knock-in mice showed suppressed beige adipocytes formation under cold stress, while the homozygous knock-in mice are cold intolerance. On the other hand, knocking out Ets1 in adipocytes enhanced energy expenditure and protect the mice from high fat diet induced metabolic disorders. Mechanical assay suggests Ets1 binds to the promoter region of mitochondria complex coding genes and autophagy related genes, transcriptionally suppresses mitochondrial biogenesis and activates its clearance. Our results indicate that Ets1 integrally regulates the balance of mitochondria generation and degradation, hence acts as a pivotal governor of mitochondria content and negatively regulates beige adipocyte formation.

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A non-cell autonomous mechanism of beige adipocyte renaissance in subcutaneous adipose tissue

Project description:Two types of UCP1 positive cells-brown and beige adipocytes exist in mammals. Beige adipocytes are very plastic, and can be dynamically regulated by environment.Beige adipocytes formed postnatally in subcutaneous inguinal white adipose tissue (iWAT) lost thermogenic gene expression and multilocular morphology at adult stage, but cold could restore their “beigeing” characteristics, a phenomenon termed as beige adipocyte renaissance. Our results showed that beige cell maintenance and renaissance in adult mice were regulated by cAMP and HDAC4 signaling in white adipocytes non-cell autonomously. Genetic modulations of various components of this cAMP-HDAC4 cascade (e.g. LKB1) led to persistent browning and reduced adiposity independent of thermogenesis. To further study the mechanisms of beige adipocytes maintenance, we performed RNA-seq with samples from inguinal white adipose tissues of WT, AdipoqCre LKB1 F/F, and AdipoqCre LKB1 F/F; HDAC4 F/F mice.Our studies will move the beige adipocyte field forward and attract clinical applications to target beige adipocyte renaissance.

2018-02-14 | GSE85335 | GEO

Transcriptomic Characterization of Human Beige Adipocyte Differentiated From Adipose Mesenchymal Stem Cells

Project description:Beige adipocytes are a distinct type of thermogenic fat cells in human. Since beige adipocytes are distributed sporadically within white adipose, characterization of human beige adipocytes has long been a problem. In this study, we reported a rapid and roboust method in generating human beige adipocyte with chemically defined medium and RNA-Seq was perfomed to reveal the molecular characterization of derived human beige adipocytes.

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