ABSTRACT: The NGS sequencing data generated in the study of PAM-flexible and sequence context-agnostic base editors for efficient and precise cytosine base editing in zebrafish
Project description:PAM-flexible and sequence context-agnostic base editors for efficient and precise cytosine base editing in zebrafish
| PRJNA1151843 | ENA
Project description:The part of NGS data of PAM-flexible and sequence context-agnostic base editors for efficient and precise cytosine base editing in zebrafish
| PRJNA1149283 | ENA
Project description:The Next Generation Sequencing data generated in the study of PAM-flexible and sequence context-agnostic base editors for efficient and precise cytosine base editing in zebrafish
Project description:The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich PAM sequences. To overcome this limitation, we developed a CRISPR/Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and converts C to T in human cells with low levels of indels, non-C-to-T substitutions and off-target editing.
