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ABSTRACT: small RNA-seq of P0 mouse testes

PROVIDER: PRJNA1041954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26867317.fastq.gz	Fastqsanger.gz
	SRR26867318.fastq.gz	Fastqsanger.gz
	SRR26867319.fastq.gz	Fastqsanger.gz
	SRR26867320.fastq.gz	Fastqsanger.gz
	SRR26867321.fastq.gz	Fastqsanger.gz

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Project description:A Comparative Study of Human Testes and Epididymis through the Proteomics and RNA-seq Methods

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Deep sequencing and high-throughput analysis of PIWI-associated small RNAs

Project description:Small RNAs are now known to be major regulatory factors of gene expression. Emerging methods based on deep-sequencing have enabled the analysis of small RNA expression in a high-throughput manner, leading to the identification of large numbers of small RNAs in various species. Moreover, profiling small RNA data together with transcriptome data enables transcriptional and post-transcriptional regulation mediated by small RNAs to be hypothesized. Here, we isolated PIWIL1 (MIWI)-associated small RNAs from mouse testes, and performed small RNA-seq analysis. In addition, directional RNA-seq was performed using Piwil1 mutant mouse testes. Using these data, we describe protocols for analyzing small RNA-seq reads to obtain profiles of small RNAs associated with PIWI proteins. We also present bioinformatic protocols for analyzing RNA-seq reads that aim to annotate expression of piRNA clusters and identify genes regulated by piRNAs.

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