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ABSTRACT: Transcriptional levels of Hemoncus contortus stimulated by host serum

PROVIDER: PRJNA1042665 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26908813_1.fastq.gz	Fastqsanger.gz
	SRR26908813_2.fastq.gz	Fastqsanger.gz
	SRR26908814_1.fastq.gz	Fastqsanger.gz
	SRR26908814_2.fastq.gz	Fastqsanger.gz
	SRR26908815_1.fastq.gz	Fastqsanger.gz

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Profiling of differentially expressed genes in sheep T lymphocytes response to Haemonchus contortus infection

Project description:Once Haemonchus contortus infects sheep it receives a series of host attacks, especially those relating to the infected animal’s T lymphocytes immune response. To obtain a systematic genome-wide profiling of the T lymphocyte genes involved, microarrays were used to compare gene expression between 0 days post infection (dpi), 3-5 dpi, 25-30 dpi and 60dpi in infected sheep. In this 853, 242 and 42 differentially expressed genes were acquired in the 3d vs. 0d comparison, the 30d vs. 0d comparison and the 60d vs. 0d comparison, respectively. Gene Ontology and pathway analysis indicated that modulated genes including SUGT1, FCER1G, CD23, IL-13 and galectin-14, were mostly associated with cellular homeostasis maintaining and immune response. Haemonchus contortus infection induced gene expression in sheep T lymphocytes was measured at 0, 3, 30 and 60 days post infection. Four time-series experiments were performed at each sheep (2#, 3# and 5#).

2012-11-16 | E-GEOD-42302 | biostudies-arrayexpress

Evaluation of serum supplementation on the development of Haemonchus contortus larvae in vitro and on compound screening results

Project description:A high-throughput platform for assessing the activity of synthetic or natural com-pounds on the motility and development of Haemonchus contortus larvae has been estab-lished for identifying new anthelmintic compounds active against strongylid nema-todes. This study evaluated the impact of serum supplementation on larval develop-ment, motility and survival in vitro and its implications for phenotypic compound screening. Of five blood components assessed, 7.5% sheep serum significantly enhanced larval development, motility and survival compared with the original medium (LB*), leading to the formulation of an improved medium (LBS*). Proteomic analysis revealed marked differences in protein expression in larvae cultured in LBS* versus LB*, includ-ing molecules associated with structural integrity and metabolic processes. The pheno-typic screening of 240 compounds (“Global Priority Box” from Medicines Malaria Ven-ture) using LBS* yielded results distinct from those in LB*, highlighting the effect of culture conditions on screening assessments. These findings indicate/emphasise the critical need to evaluate and optimise culture media for physiologically conditions in screening platforms, improving the reliability of anthelmintic discovery.

2025-02-27 | PXD060092 | Pride

MicroRNA microarray of Haemonchus contortus lifecycle stages and gut tissue

Project description:Examined expression of microRNAs in different life-cycle stages and gut tissue of the pathogenic nematode Haemonchus contortus and identified differentially expressed miRNAs. Custom array contained probes to 609 Haemonchus contortus miRNA sequence (all mature and start miRNAs identified by Winter et al., 2012, BMC Genomics13:4; DOI: 10.1186/1471-2164-13-4) and all C. elegans sequences in miRBase release 16

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Project description:Illumina sequencing of small RNAs from Brugia pahangi and Haemonchus contortus

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Profiling of differentially expressed genes in sheep T lymphocytes response to Haemonchus contortus infection

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High-throughput sequencing of small RNAs from Brugia pahangi and Haemonchus contortus

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Project description:Haemonchus contortus

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Proteomic analysis of dafachronic acid-associated alterations in Haemonchus contortus

Project description:To investigate the molecular changes (protein level) associated with dafachronic acid and the nuclear hormone receptor DAF-12 in Haemonchus contortus following exsheathment of the thrid-stage larvae, protein was extracted from replicates (n = 4), processed and analysed using LC-MS/MS.

2019-11-13 | PXD012878 | Pride

Haemonchus contortus transcriptome

Project description:Haemonchus contortus is a highly pathogenic parasitic nematode of that can infect a large number of wild and domesticated ruminant species and is the most economically important parasite of sheep and goats worldwide. Although originally a tropical parasite, it has been disseminated around the world by livestock movement and can now be found as far north as the arctic circle. Adult worms are blood feeders that reside in the abomasum (stomach) and are approximately 2cm in length when mature. They are dioecious with single females typically producing several thousand eggs per day which pass out of the host in faeces and develop to infective larvae on the pasture. H. contortus is a member of the superfamily trichostrongyloidea (Strongylida) which contains most of the economically important parasitic nematodes of grazing livestock. These parasites cost the global livestock industry billions of dollars per annum in lost production and drug costs. Resistance to all the major anthelmintic classes is now common worldwide often leading to failure of treatment and control. H. contortus is a close relative of the human hookworm species and belongs to the nearest phylogenetic group of parasites to the free-living model nematode Caenorhabditis elegans . This makes it an important model of parasitic nematode biology that is commonly used for experimental studies. The main objective of this project is to recognize genes expressed in the life stages of H. contortus.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-02-12 | E-ERAD-143 | biostudies-arrayexpress

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Project description:Haemonchus contortus overview

| PRJNA193279 | ENA