Project description:21 DPI Marchantia polymorpha Tak-1 thalli were infected with Fol WT and erc3 knock out alongside mock(water) control and axenically grown Fol WT culture. At 3 days post infection, thalli were harvested and RNA was extracted. After polyA enrichment, and library preparation, sequencing was carried on NovaSeq6000 platform using SP flowcell with 2 x 100 bp read length.
Project description:The growth orientation of the Marchantia polymorpha thallus – a system of dorsiventralized, indeterminate axes – is modulated by light. We show that red and blue light act antagonistically to control thallus flatness, with red light signalling promoting epinasty and blue light signalling promoting hyponasty. We found that loss-of-function mutations in the blue light receptor MpPHOT led to epinasty, while loss-of-function mutations in the red light receptor MpPHY resulted in hyponasty. We hypothesize that these antagonistic activities of blue and red light signalling are balanced in white light, resulting in the development of flat thalli. Using time-resolved transcriptomics, we identified genes that were rapidly induced upon light exposure. Among these genes were all six members of the M. polymorpha BBX gene family. Mutants harbouring loss-of-function mutations in two of the six MpBBX transcription factors developed defective thalli. Mpbbx1 loss-of-function mutants formed hyponastic thalli, while Mpbbx5 loss-of-function mutants developed epinastic thalli. Double mutants Mpbbx1 Mpbbx5 grew flat, supporting the hypothesis that they function antagonistically. Together, these data indicate that phototropin-mediated blue light and phytochrome-mediated red light signalling antagonistically modulate thallus flatness, and that BBX transcription factors also act antagonistically to regulate thallus flatness.
Project description:To investigate gene expression in different cells of Chara braunii we performed RNA sequencing of whole thali with rhizoids and thalli without rhizoids in strains NIES 1604 and S276.
Project description:RNA was extracted from axenically grown Fo47 and Fol cultures and Marchantia polymorpha Tak1 thalli infected with these strains at 3DPI. There are 3 biological replicates in each set. Sequencing was carried out using NovaSeq 6000 platform using SP flowcell with 2x100bp sequencing read length.
Project description:Thalli of calcified red macroalga A. fragilissima were grown at different pCO2 conditions; low, medium, and high pCO2 (400, 1,000 and 1,600 ppm).
Project description:Thalli of calcified red macroalga Halimeda opuntia were grown at different pCO2 conditions; low, medium, and high pCO2 (400, 1,000 and 1,600 ppm).
Project description:Thalli of calcified red macroalga Amphiroa fragilissima and green macroalga Halimeda opuntia were grown at different light conditions (30, 150 and 300 µmol photons m-2 s-1).