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Spatial profiling of synovial tissue from patients with late-stage knee osteoarthritis.

ABSTRACT: Spatial profiling of synovial tissue from patients with late-stage knee osteoarthritis.

PROVIDER: PRJNA1044060 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26918800_1.fastq.gz	Fastqsanger.gz
	SRR26918800_2.fastq.gz	Fastqsanger.gz
	SRR26918900_1.fastq.gz	Fastqsanger.gz
	SRR26918900_2.fastq.gz	Fastqsanger.gz
	SRR26918801_1.fastq.gz	Fastqsanger.gz

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Spatial profiling of synovial tissue from patients with late-stage knee osteoarthritis.

Project description:Marked changes in synovial tissue are seen in knee osteoarthritis and are related to pain; however, the regulation of these processes are not fully understood. Spatial profiling was used to characterize cellular profiles and biological processes within important microenvironments in the synovium (intimal lining, sub-lining, and vessels) between patients that experience more pain compared to those that experience less pain.

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2011-11-01 | E-GEOD-32317 | biostudies-arrayexpress

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Project description:The underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins on a global level in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA. Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e. interplay) between the proteins in each group. More proteins were increased in early-OA vs controls than late-stage OA vs controls. For most of these proteins, the fold changes between late-stage OA vs controls and early stage OA vs controls were remarkably similar suggesting potential involvement in the OA process. Further, for the first time this study illustrated distinct patterns in protein co-expression suggesting that the global interplay between the protein machinery is increased in early-OA and lost in late-stage OA. Further efforts should probably focus on earlier stages of the disease than previously considered.

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Single cell RNA-seq of human knee osteoarthritis synovium

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