Project description:Disturbing the biosynthetic pathway of 17-hydroxylgeranyllinalool diterpene glycosides (HGL-DTGs), including silencing NaCYP736A, caused severe plant autotoxicity. This autotoxicity can be restored by co-silencing the upstream biosynthetic genes, like geranyllinalool synthase (GLS). Through comparing the transcriptome data of EV, VIGS-GLS, VIGS-CYP736A and VIGS-GLS&CYP736A, we tried to figure out the potential mechanism of this autotoxicity.
Project description:Here, we profiled the transcriptional capacity of a library of regulatory sequences mined from diverse Biosynthetic Gene Clusters in S. albidoflavus (S. albus J1074) to investigate BGC gene regulation.
Project description:Stevia rebaudiana (stevia) is a plant that accumulates high levels of steviol glycosides (SGs) in its leaves, which provide strong sweetness. In this study, we used single‑nucleus RNA sequencing (snRNA‑seq) to resolve the expression landscape of SG‑biosynthetic genes across individual cell types in stevia leaves. We identified distinct nuclear clusters corresponding to different cell types such as mesophyll, epidermis, trichomes and vascular bundle sheath. Notably, UGT76G‑family and UGT91D4 sugar‑transferase genes showed high expression in mesophyll and epidermal nuclei, suggesting spatial division of SG production within leaf tissues.
Project description:Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Lysosomes are membrane-bound organelles critical for maintaining cellular homeostasis. Delivery of biosynthetic lysosomal proteins to lysosomes is crucial to orchestrate proper lysosomal function. However, it remains unknown how the delivery of biosynthetic lysosomal proteins to lysosomes is ensured in neurons, which are highly polarized cells. Here, we developed Protein Origin, Trafficking And Targeting to Organelle Mapping (POTATOMap), by combining trafficking synchronization and proximity-labelling based proteomics, to unravel the trafficking routes and interactome of the biosynthetic lysosomal membrane protein LAMP1 at specified time points.
Project description:Data files and supplementary data associated with the one-pot method. These files are the results of one-pot enrichment of both acetylated and succinylated peptides.
2017-12-08 | MSV000081794 | MassIVE
Project description:Temperature Control-Enabled Programmable One-Pot Polymerase-Mediated DNA Synthesis
