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Laser microdissection coupled with RNA-seq profiling of the E11.5 mouse mandibular epithelium along the dorsoventral axis

ABSTRACT: Laser microdissection coupled with RNA-seq profiling of the E11.5 mouse mandibular epithelium along the dorsoventral axis

PROVIDER: PRJNA1047608 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27019000_1.fastq.gz	Fastqsanger.gz
	SRR27019000_2.fastq.gz	Fastqsanger.gz
	SRR27019001_1.fastq.gz	Fastqsanger.gz
	SRR27019001_2.fastq.gz	Fastqsanger.gz
	SRR27019002_1.fastq.gz	Fastqsanger.gz

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Laser microdissection coupled with RNA-seq profiling of the E11.5 mouse mandibular epithelium along the dorsoventral axis

Project description:How the dorsal-ventral axis of the vertebrate jaw, particularly the position of tooth initiation site, is established remains a critical and unresolved question. Tooth development starts with the formation of the dental lamina, a localized thickened strip within the maxillary and mandibular epithelium. To identify transcriptional regulatory networks (TRN) controlling the specification of dental lamina from the naïve mandibular epithelium, we utilized Laser Microdissection coupled low-input RNA-seq (LMD-RNA-seq) to profile gene expression of different domains of the mandibular epithelium along the dorsal-ventral axis in wild type mouse and two loss-of-function mouse models of domain specific transcription factors.

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