Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1047677 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In the present study we have assessed, by transcriptional profiling, the systemic defense response of Zea mays plants to the ear rotting pathogen Fusarium verticilioides induced by the beneficial fungus Trichoderma atroviride
2020-01-13 | GSE93760 | GEO
Project description:Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained small RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata. The libraries were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC).
2022-02-28 | GSE197448 | GEO
Project description:Trichoderma species promote growth and strengthen immunity of Arabidopsis and crop species through multiple mechanisms. However, how fungal proteins mediate growth-defense tradeoffs is unknown. We analyzed the growth, root architecture, defense and global gene expression profiles in Arabidopsis seedlings co-cultivated with T. atroviride WT, and Δnox1, Δnox2, and ΔnoxR mutants, defective on the catalytic and regulatory subunits of NADPH oxidase, respectively. The gene expression profile in the fungus was also characterized in standard growth conditions and in the presence of plants. The results revealed the critical role of Trichoderma NoxR in mediating growth-defense tradeoffs in Arabidopsis. The effects of T. atroviride WT in improving root branching and biomass production decreased in all three related NADPH defective mutants, particularly in ΔnoxR. In contrast, induction of jasmonic acid-related defense responses in roots and shoots were exacerbated in ΔnoxR compared to the WT strain. Transcriptome analyses showed a tight plant-fungus communication based on reactive oxygen species and availability of carbon resources. The ΔnoxR is unable to perceive changes in nutrient sources and activate signaling cascades, which suppresses the metabolic change from saprophyte to commensal. Thus we conclude that Trichoderma NoxR orchestrates fungal-induced development and defense tradeoffs in Arabidopsis and plays an important role in cross-kingdom plant-fungus communication.
2019-10-01 | GSE138203 | GEO
Project description:This study investigates the multifaceted defense mechanisms of plants in response to beneficial fungi Trichoderma atroviride. The researchers focus on a small RNA, Ta_sRNA1, produced by T. atroviride during its interaction with Arabidopsis thaliana.
2024-03-29 | GSE254275 | GEO
Project description:Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata and Rhizoctonia solani. The transcriptomes were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC).
2021-12-05 | GSE190033 | GEO
Project description:Trichoderma species can stimulate local and distant immune responses in colonized plant tissues to prevent future pathogenic attacks. Priming of plant defenses is characterized by changes on transcriptional, metabolic, and epigenetic states after stimulus perception. We have previously investigated the transcriptional reprogramming in silk tissues from maize plants inoculated with Trichoderma atroviride and challenged with Fusarium verticillioides (Agostini et al 2019). To better understand about the molecular changes induced by T. atroviride in maize, a proteomic approach was conducted in this instance. Several proteins belonging to different metabolic categories were detected as priming involved proteins. However, we detected a very low correlation with those priming-modulated transcripts suggesting the importance of regulatory events posteriori of transcriptional stage to accomplish the final goal of blocking the pathogen entrance. Specifically, we focused on phenylpropanoid pathway; since we detected several proteins that are upregulated in priming state might explain the cell wall reinforcement and, the increase in the content of flavonoids and lignin in silks of maize plants after induced systemic resistance activation.
2021-05-03 | PXD024698 | Pride
Project description:Phosphite (Phi) is widely used in agriculture due to its biostimulant effects on plants and its ability to control various phytopathogens. However, its impact on beneficial soil microorganisms remains poorly understood. In this study, we evaluated the effect of Phi on the growth and transcriptional response of the beneficial fungus Trichoderma atroviride. Our results show that low concentrations of Phi, in the presence of phosphate (Pi), promote the growth of T. atroviride, whereas higher concentrations inhibit its development. Transcriptomic analysis via RNA-Seq revealed the activation of genes associated with growth, amino acid biosynthesis, and siderophore transport. Furthermore, Phi enhanced the antagonistic capacity of T. atroviride against Rhizoctonia solani. These findings reveal a novel role of Phi in stimulating beneficial fungi and suggest its combined use with T. atroviride as a sustainable strategy for phytopathogen biocontrol in agricultural systems.
2025-12-18 | GSE308028 | GEO