Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1047940 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27028809_1.fastq.gz | Fastqsanger.gz | |||
| SRR27028809_2.fastq.gz | Fastqsanger.gz | |||
| SRR27028810_1.fastq.gz | Fastqsanger.gz | |||
| SRR27028810_2.fastq.gz | Fastqsanger.gz | |||
| SRR27028811_1.fastq.gz | Fastqsanger.gz |
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Project description:Soil salinization-alkalization is a widespread abiotic stress globally, severely impairing crop growth and environmental ecology. Nanomaterials hold significant potential for application in regulating plant stress tolerance due to their unique physical and chemical properties. In this study, the sol-gel method was optimized to synthesize a type of spherical, monodisperse, amorphous mesoporous silica nanoparticles (MSNs) with an average particle size of 40-50 nm and a positive surface charge of 35 mV. A 20 mg/L MSN solution was foliarly applied to two soybean cultivars, Hefeng 50 (HF50) and Henong 95 (HN95), in a pot experiment at the R1 stage to investigate its effects on plant growth and yield-related traits under saline-alkali stress (pH 9.2). MSNs treatment significantly alleviated the growth inhibition induced by saline-alkali stress. Specifically, MSNs significantly increased the plant height, leaf area, root volume, and root surface area of soybean plants under saline-alkali stress. MSNs also increased the dry matter accumulation of plant shoots and roots, improved root activity, and increased the number of pods and seeds per plant. MSNs enhanced cell mechanical strength by activating the synthesis of cuticle and epidermal wax, and maintained intracellular metabolic homeostasis by activating the antioxidant defense system and suppressing the excessive activation of pathways such as the mitogen-activated protein kinase (MAPK) pathway. Additionally, MSNs increased the content of photosynthetic pigments in soybean leaves, thereby enhancing light energy absorption and utilization efficiency, and elevating carbohydrate levels. MSNs promoted the directed translocation of photosynthates to sink organs such as seeds and roots by regulating the activity of carbon metabolism enzymes and source-sink allocation. Taken together, these cumulative effects improved soybean saline-alkali stress tolerance, and ultimately increasing the number of pods per plant, seeds per plant, 100-seed weight, and total yield per plant. The study provides theoretical basis and technical support for the development of nanomaterial-based cultivation practices to improve crop saline-alkali tolerance.
2026-01-20 | GSE316965 | GEO
Project description:Alkali stress is one of the most severe abiotic stresses affecting agricultural production worldwide. To understand the phosphorylation events in soybean in response to alkali stress, we performed the TMT labeling-based quantitative phosphoproteomic analyses on soybean leaf and root tissues under 50 mM NaHCO3 treatment.
2024-01-26 | PXD041842 | Pride
Project description:Salt stress, especially saline-alkali stress, has seriously negative effect on citrus production. Ziyang xiangcheng (Citrus junos Sieb.) (Cj) has been reported as a saline-alkali stress and iron deficiency tolerant citrus rootstock. However, the molecular mechanism of its saline-alkali stress tolerance is still not clear. Two citrus rootstocks and one navel orange scion, Cj, Poncirus trifoliate (Poncirus trifoliata (L.) Raf.) (Pt) and ‘Lane Late’ navel orange (Citrus sinensis (L.) Osb.) (LL), were used in this study. The grafted materials Cj+LL and Pt+LL grown in calcareous soil were used to identify genes and pathways responsive to saline-alkali stress using RNA-seq. The seedlings of Cj and Pt grown in the solutions with different gradient pH value were used to perform a supplement experiment. Comprehensively analyzing the data of RNA-seq, physiology and biochemistry, agronomic traits and mineral elements of Cj+LL, Pt+LL, Cj and Pt, several candidate pathways and genes were identified to be highly regulated under saline-alkali stress. Here, we propose citrate is important for the tolerance to iron deficiency and the jasmonate (JA) biosynthesis and signal transduction pathway may play a crucial role in tolerance to saline-alkali stress in citrus by interacting with other plant hormones, calcium signaling, ROS scavenging system and lignin biosynthesis.
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Project description:<p>Metabolic data of the first leaf (L1) and the third leaf (L3) of two oat (Avena sativa) cultivars: DY5 (stress sensitive) and BY14 (stress tolerant) at 52 days post sowing (DPS) under saline-alkali stress (implicated at 37 DPS).</p>
2025-10-20 | MTBLS13180 | MetaboLights
Project description:Soil salination and alkalization are global problems impairing plant survival by disrupting REDOX homeostasis. Whether melatonin regulates REDOX homeostasis at nitrosative level, and thus affects plant saline-alkali tolerance remains unknown. In saline-alkali stress, excess nitric oxide (NO) causes nitrosative damage in tomato roots. This NO can be degraded by S-nitrosoglutathione reductase (GSNOR), or stimulates caffeic acid O-methyltransferase (COMT) transcript for melatonin synthesis. Melatonin further feedback scavenges excess NO to alleviate nitrosative damage at the whole protein level, indicating by proteome S-nitrosylation. We target plasma membrane H+-ATPase 2 (HA2) and highlight that HA2 is S-nitrosylated at Cys206 in saline-alkali stress, reducing HA activity, H+ efflux, and tolerance by impairing its interaction with 14-3-3 protein 1 (TFT1). In agreement with these observations, COMT-mediated melatonin relieves the HA2 S-nitrosylation to recover its function and saline-alkali tolerance. Therefore, we propose NO and melatonin as a pair of REDOX switches to control HA2 S-nitrosylation and saline-alkali tolerance. Under natural saline-alkali conditions, tomato productivity can be improved by grafting with COMT-, GSNOR-, HA2-overexpression rootstocks or by genetic engineering non-nitrosylated HA2C206S mutants. Using melatonin-NO-HA2 module as a case, this study illuminates a novel molecular function of melatonin and relevant genetic engineering strategies in future agriculture.
2025-04-28 | PXD056815 | Pride
Project description:Purpose:Salinity is an important environmental factor that affects the physiological activities of fish. The goals of this study are investigating the effect of different saline-alkali stress on grass carp (Ctenopharyngodon idella). Methods: Grass carp individuals, averaging 12 cm in body length, were obtained from Duofu fish farm (Wuhan, China) and cultured at recirculating aquaculture system for 2 weeks before the experiment began. For the challenge, all grass carp were randomly divided into three groups, and then cultured at saline-alkali water with the concentration of 0, 3‰ and 6‰. After 30 days, some grass crap cultured at 3‰ and 6‰ saline-alkali water were injured. At the same time, gill samples of grass carp were collected from 0, 3‰ (grass carp was not injured), 3‰ (grass carp was injured), 6‰ (grass carp was not injured) and 6‰ (grass carp was injured)saline-alkali groups. Total RNA of all samples was isolated using TRIzol® Reagent (Invitrogen) according to the manufacturer's introduction. RNA integrity was assessed using an Agilent 2100 bioanalyzer (Agilent, USA). Samples with RNA integrity numbers (RINs) ≥ 7.5 were subjected to cDNA library construction using TruseqTM RNA sample prep Kit (Illumina). Results:A total of 15 were processed for transcriptome sequencing, generating 94.99Gb Clean Data. At least 5.76Gb clean data were generated for each sample with minimum 91.87% of clean data achieved quality score of Q30. Clean reads of each sample were mapped to specified reference genome. Mapping ratio ranged from 88.59% to 92.84%. The expression of genes was quantified and differentially expressed genes were identified based on their expression.Criteria for differentially expressed genes was set as Fold Change(FC)≥1.5 and Pvalue<0.05. Fold change(FC) refers to the ratio of gene expression in two samples. These DEGs were further processed for functional annotation and enrichment analysis. Conclusions: Our study represents Effects and molecular regulation mechanisms of saline-alkali stress on the healthy grass carp by using RNA-seqtechnology. Our results show that saline-alkali stress will impair the immune system of grass carp.
2021-12-31 | GSE185170 | GEO
Project description:Isoprene, a volatile hydrocarbon, is typically emitted from the leaves and other aboveground plant organs; isoprene emission from roots is not well studied. Given its well-known function in plant growth and defense aboveground, isoprene may also be involved in shaping root physiology to resist belowground stress. We used isoprene-emitting transgenic lines (IE) and a non-emitting empty vector and/or wild type lines (NE) of Arabidopsis to elucidate the roles of isoprene in root physiology and salt stress resistance. We assessed root phenotype and metabolic changes, hormone biosynthesis and signaling, and stress-responses under normal and saline conditions of IE and NE lines. We also analyzed the root transcriptome in the presence or absence of salt stress. IE lines emitted isoprene from roots, which was associated with higher primary root growth, root biomass, and root/shoot biomass ratio under both control and salt stress conditions. Transcriptome data indicated that isoprene altered the expression of key genes involved in hormone metabolism and plant responses to stress factors. Our findings reveal that root constitutive isoprene emission sustains root growth also under salinity by regulating and/or priming hormone biosynthesis and signaling mechanisms, amino acids biosynthesis, and expression of key genes relevant to salt stress defense.
2024-06-25 | GSE270516 | GEO