Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LPS stimulation of APOE knock-out iPSC-derived microglia with different ApoE isoforms

ABSTRACT: LPS stimulation of APOE knock-out iPSC-derived microglia with different ApoE isoforms

PROVIDER: PRJNA1052733 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27213873.fastq.gz	Fastqsanger.gz
	SRR27213874.fastq.gz	Fastqsanger.gz
	SRR27213875.fastq.gz	Fastqsanger.gz
	SRR27213876.fastq.gz	Fastqsanger.gz
	SRR27213877.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

LPS stimulation of APOE knock-out iPSC-derived microglia with different ApoE isoforms

Project description:APOE is the main genetic modifier for late onset Alzheimer’s disease (LOAD). While an APOE2/APOE3/APOE4 allelic series is well established for LOAD risk and neuropathology, molecular mechanisms underlying isoform-dependent risk and relevance of ApoE-associated lipids remain elusive. Here, we studied the effects of LPS stimulation on APOE KO iPSC-derived microglia treated with different ApoE isoforms (ApoE2/E3/E4) pre-complexed with BODIPY-cholesteryl ester (CE) and HDL -/+ recombinant LDLR extracellular domain.

2024-11-10 | GSE250249 | GEO

ApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigation

Project description:U87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue.

2026-06-30 | E-MTAB-16242 | biostudies-arrayexpress

Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS)

Project description:We have employed whole genome microarray expression profiling to identify differently expressed genes following stimulation of interleukin-16-knock-out bone-marrow derived macrophages with Escherichia coli lipopolysaccharide (LPS). Macrophages were stimulated with LPS (100 ng/ml) for 6 hours and a signature was identified that distinguished between infected and control samples. Expression of several genes from this signature was quantified in the same RNA samples by real-time PCR, confirming the predicted macrophage response pattern.

2010-10-08 | GSE20208 | GEO

Expression data from wt, Lkb1 conditional knock out and LPS treated DC

Project description:Expression data from wt, Lkb1 conditional knock out and LPS treated DC

| PRJNA481663 | ENA

ApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigation

Project description:ApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigation

| PRJEB104168 | ENA

Murine bronco alveolar lavage fluid from wild type and mannose receptor knock out mice, unchallenged and LPS challenged

Project description:A comparative analysis of the protein content in bronco alveolar lavage fluid from wild type and mannose receptor knock-out littermate mice. This includes both an unchallenged mice and mice subjected to intratracheal LPS instillation to induce injury and inflammation.

2024-04-17 | PXD049201 | Pride

The APOE isoforms differentially shape the transcriptomic and epigenomic landscapes of human microglia in a xenotransplantation model of Alzheimer’s disease [RNA-seq]

Project description:Microglia play a key role in the response to amyloid beta in Alzheimer’s disease (AD). In this context, the major transcriptional response of microglia is the upregulation of APOE, the strongest late-onset AD risk gene. Of its three isoforms, APOE2 is thought to be protective, while APOE4 increases AD risk. We hypothesised that the isoforms functionally alter microglia by shaping their transcriptomic and chromatin landscapes. We used RNA- and ATAC-sequencing to profile gene expression and chromatin accessibility of human microglia isolated from a xenotransplantation model of AD. We identified widespread transcriptomic and epigenomic differences which are dependent on APOE genotype, and are corroborated across the profiling assays.

2024-07-08 | GSE271385 | GEO

The APOE isoforms differentially shape the transcriptomic and epigenomic landscapes of human microglia in a xenotransplantation model of Alzheimer’s disease [ATAC-seq]

Project description:Microglia play a key role in the response to amyloid beta in Alzheimer’s disease (AD). In this context, the major transcriptional response of microglia is the upregulation of APOE, the strongest late-onset AD risk gene. Of its three isoforms, APOE2 is thought to be protective, while APOE4 increases AD risk. We hypothesised that the isoforms functionally alter microglia by shaping their transcriptomic and chromatin landscapes. We used ATAC- and ATAC-sequencing to profile gene expression and chromatin accessibility of human microglia isolated from a xenotransplantation model of AD. We identified widespread transcriptomic and epigenomic differences which are dependent on APOE genotype, and are corroborated across the profiling assays.

2024-07-08 | GSE271384 | GEO

Transcription profiling of aortae from 32 week old apoE knock-out and wild type mice

Project description:Aortae of 32 weeks old apoE mice versus wild type mice on a C57BL/6J Background.

2008-09-05 | E-GEOD-2372 | biostudies-arrayexpress

Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS)

2010-10-08 | E-GEOD-20208 | biostudies-arrayexpress