Spiroplasma eriocheiris
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ABSTRACT:
PROVIDER: PRJNA1053828 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Spiroplasma eriocheiris Raw sequence reads
| PRJNA1053831 | ENA
Project description:The raw date of Ubiquitin-modified proteome analysis of Eriocheir sinensis hemocytes during Spiroplasma eriocheiris infection
2021-11-25 | PXD029908 |
Project description:Spiroplasma eriocheiris strain:DSM 21848 Genome sequencing
| PRJNA253647 | ENA
Project description:Spiroplasma eriocheiris CCTCC M 207170 Genome sequencing
| PRJNA46263 | ENA
Project description:Spiroplasma eriocheiris, has been identified as a novel lethal pathogen of Eriocheir sinensis tremor disease (TD), one neurological disease with typically paroxysmal tremors of the pereiopod. This pathogen infected and multiplied in the hemocytes of E. sinensis as the first target cells, and then follow the blood circulation to infect the crab other tissues. S. eriocheiris infected the nerves tissue is the directly reason of TD. But the pathogenic mechanism of TD was still few known. Firstly, in the current study, the phosphoproteomic changes of E. sinensis thoracic ganglion after S. eriocheiris infection were obtained. KEGG pathway analysis show Wnt signaling pathways was restrained, corresponding many nervous system development and signal transmission pathway also destroyed. Base on the identified modified sequence, several peptides (GSK3β, SYN, VAMP and SNAMP-25) were selected to synthesize chemically and prepare phosphorylated antibodies. The differentially expressed phosphorylated proteins GSK3β-pSer9, VAMP-pSer72, SNAP25-pSer102, and SYN-pSer134 in thoracic ganglion were significantly down-regulated verified by immunohistochemistry and western blot, this results are similar with the phosphoproteomic. By qRT-PCR, western blot, RNA interference and inhibitor experiments, when the S. eriocheiris infected the hemocytes of crab, the GSK-3β and β-Catenin in Wnt-β-Catenin pathway were restrained similar in the thoracic ganglion. The S. eriocheiris can restricts the hemocytes Wnt-β-Catenin pathway to help itself infection no matter in vivo or in vitro. Neurotransmitter metabolite analysis showed that four kinds of neurotransmitters (5-Hydroxy-L-tryptophan, Serotonin, Acetylcholine and γ-Amino-butyric acid) in thoracic ganglion were metabolic disorders in E. sinensis thoracic ganglion after S. eriocheiris infection. The present work could serve as a basis for understanding the role of Wnt signaling pathway in the process of S. eriocheiris invasion E. sinensis hemocytes and causing paroxysmal tremors of E. sinensis the pereiopod.
2025-10-06 | PXD022892 | Pride
Project description:Endosymbiotic bacteria associated with eukaryotic hosts are omnipresent in nature, particularly in insects. Studying the bacterial side of host-symbiont interactions is, however, often limited by the unculturability and genetic intractability of the symbionts. Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with several Drosophila species. S. poulsonii strongly affects its host’s physiology, for example by causing male killing or by protecting it against various parasites. Despite intense work on this model since the 1950s, attempts to cultivate endosymbiotic Spiroplasma in vitro have failed so far. Here, we developed a method to sustain the in vitro culture of S. poulsonii by optimizing a commercially accessible medium. We also provide a complete genome assembly, including the first sequence of a natural plasmid of an endosymbiotic Spiroplasma species. Last, by comparing the transcriptome of the in vitro culture to the transcriptome of bacteria extracted from the host, we identified genes putatively involved in host-symbiont interactions. This work provides new opportunities to study the physiology of endosymbiotic Spiroplasma and paves the way to dissect insect-endosymbiont interactions with two genetically tractable partners.
2018-03-24 | GSE112290 | GEO
Project description:Morus alba L. Raw protein sequence reads and sequence
2024-02-08 | PXD049451 |
Project description:We investigated the effect of Spiroplasma infection on Drosophila hemolymph protein content using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). To this end, we extracted total hemolymph from uninfected and infected 10 days old females. At this age, Spiroplasma is already present at high titers in the hemolymph but does not cause major deleterious phenotypes to the fly. Extraction was achieved by puncturing the thorax and drawing out with a microinjector. Four replicates were made
2021-05-04 | PXD024340 | Pride