The role of chorionic PGRMC2 and HLA-G in maintaining immune homeostasis at the chorio-decidual interface
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1055482 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27318500.fastq.gz | Fastqsanger.gz | |||
| SRR27318501.fastq.gz | Fastqsanger.gz | |||
| SRR27318502.fastq.gz | Fastqsanger.gz | |||
| SRR27318503.fastq.gz | Fastqsanger.gz | |||
| SRR27318504.fastq.gz | Fastqsanger.gz |
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Project description:The chorio-decidual interface (CDi) is formed by chorion trophoblasts (CTCs) and immune cell-rich decidua (DECs) cells. The roles of human leukocyte antigen (HLA)-G and progesterone receptor membrane component 2 (PGRMC2) in mediating innate immune homeostasis at this maternal-fetal interface interface were investigated.
2024-01-02 | GSE251809 | GEO
Project description:Deciphering the immune privilege of maternal-fetal interface is critical to understand how reproductive success and host defense are simultaneously maintained. Here, we identify a conserved subset of effector-like memory CD8⁺ T cells that are preferentially infiltrate in the decidua of both humans and mice, via the CX3CL1–CX3CR1 axis. Murine decidual CX3CR1⁺ memory CD8⁺ T cells and human decidual CD45RA⁺ CD8⁺ T cells share transcriptional and functional programs, characterized by reduced cytokine output but enhanced cytotoxic granule production. We show that decidual stromal cells upregulate CX3CL1 during decidualization, which recruits CX3CR1⁺ cells via receptor engagement and internalization. Functionally, these cells mediate enhanced local protection against Listeria monocytogenes infection at the fetal-maternal interface in a CX3CL1-dependent manner, without contributing to fetal damage. These findings define a conserved mechanism by which decidual tissues selectively enrich effector-like memory CD8⁺ T cells, enabling localized pathogen surveillance while maintaining maternal–fetal tolerance.
2026-09-04 | GSE307408 | GEO
Project description:Congenital cytomegalovirus (cCMV) is the most common intrauterine infection, leading to infant neurodevelopmental disabilities. An improved knowledge of correlates of protection against cCMV is needed to guide prevention strategies. Here, we employed a unique ex vivo model of human CMV (HCMV) infection in decidual tissues of women with and without preconception immunity, recapitulating nonprimary versus primary infection at the authentic maternofetal transmission site. We showed that decidual tissues of women with preconception immunity exhibited intrinsic resistance to HCMV, mounting a rapid activation of tissue resident memory CD8+ and CD4+ T cells upon nonprimary infection. We further revealed the role of HCMV-specific decidual-tissue resident CD8+ T cells in local protection against nonprimary HCMV infection. The findings could inform the development of vaccine against cCMV, and provide insights for further studies of the integrity of immune defense against cCMV and other pathogens in the human maternal-fetal interface.
2024-08-01 | GSE240976 | GEO
Project description:Maternal and fetal monocytes and tissue macrophages (decidual macrophages, Hofbauer cells) at the feto-maternal interface have different methylome. Paired and balanced design. We compared maternal blood monocytes (MB) vs. cord blood monocytes (CB), maternal blood monocytes (MB) vs. decidual macrophages (Deci), cord blood monocytes (CB) vs placental macrophages (villi) and decidual macrophages (Deci) vs. placental macrophages (villi).
2011-11-15 | E-GEOD-31680 | biostudies-arrayexpress
Project description:The initiation of human labour is associated with inflammatory activation at the maternal-fetal interface; however, the cellular mechanisms driving this process remain incompletely understood. Decidual macrophages are abundant leukocytes within the decidua and are thought to play important roles in maintaining pregnancy and promoting labour-associated inflammation. Despite this, macrophage heterogeneity within the decidua remains poorly defined, with multiple classification systems proposed but no consensus regarding their biological relevance or application across gestation and labour. This thesis investigates decidual macrophage heterogeneity and function in decidua parietalis using flow cytometry, bulk RNA sequencing, RT-qPCR, ELISA, and functional tissue culture assays. Initial studies sought to validate a labour-associated macrophage population identified by single-cell RNA sequencing. Although a population with a similar phenotype was identified by flow cytometry, its extremely low abundance suggested it was unlikely to be a major driver of labour-associated immune adaptation. This prompted a broader investigation of decidual macrophage phenotyping strategies. Current and historical macrophage classification systems, including M1/M2 and several CD11c-based frameworks, failed to reliably distinguish macrophage subsets within this dataset. In contrast, a phenotyping strategy incorporating CD14, pan macrophage marker CD68, and tissue residency marker FOLR2 consistently identified monocytes, FOLR2- macrophages, and FOLR2+ macrophages across first trimester and term decidua, within both decidua parietalis and decidua basalis, and across term not in labour (TNL), term early labour (TeaL), and term in labour (TIL) samples. Bulk RNA sequencing validated FOLR2- and FOLR2+ macrophages as transcriptionally distinct populations. FOLR2- macrophages were enriched for pathways associated with neutrophil chemotaxis, antimicrobial responses, and inflammation, whereas FOLR2+ macrophages were enriched for antigen presentation, MHC class II assembly, and tissue-resident functions. Labour was associated with selective remodelling of the decidual myeloid compartment rather than global leukocyte infiltration. Monocyte abundance increased significantly during early labour, while the proportion of FOLR2- macrophages decreased before increasing again in established labour. Functional analyses demonstrated distinct cytokine profiles between macrophage subsets. FOLR2- macrophages preferentially expressed IL-8 and increased CCL5 expression during early labour, consistent with a chemotactic and inflammatory phenotype. In contrast, FOLR2+ macrophages exhibited high expression of IL-6, CCL2, MMP-9, and MMP-19, supporting roles in tissue homeostasis, leukocyte recruitment, and extracellular matrix remodelling. Furthermore, co-culture experiments demonstrated that FOLR2+ macrophages promoted degradation of chorionic collagen in vitro. Together, these findings support a model in which labour-associated immune adaptation is mediated by functionally specialised decidual macrophage populations rather than a single labour-specific macrophage subset. Functional analysis of FOLR2- macrophages suggests they contribute predominantly to inflammatory signalling and leukocyte recruitment, whereas FOLR2+ macrophages contribute to antigen presentation and tissue remodelling. This work establishes a simple and robust framework for decidual macrophage phenotyping that is applicable across gestation, decidual compartment, and labour status, providing a foundation for future studies investigating macrophage function at the maternal-fetal interface.
2026-07-02 | GSE337384 | GEO
Project description:Receptors of the KIR family on maternal Natural Killer cells in the decidua are believed to bind to Ligands such as HLA-C on trophoblast cells invading into the decidua from the placenta during early pregnancy. The resulting responses regulate the extent of trophoblast invasion. Genetic studies have implicated two members of the KIR family in particular as playing an important role: KIR2DL1 and KIR2DS1. The aim of this experiment was to determine what responses are generated when decidual NK cells bearing either KIR2DL1 or KIR2DS1 engage their HLA-C ligand.
2014-04-04 | E-MTAB-1784 | biostudies-arrayexpress
Project description:A Toxoplasma gondii infection during pregnancy can result in spontaneous abortion, preterm labor, or congenital fetal defects. The decidual immune system plays a critical role in regulating the immune micro-environment and in the induction of immune tolerance. To better understand the factors that mediate the decidual immune response associated with the T. gondii infection, a large-scale study employing TMT proteomics was conducted to characterize the differential decidual immune proteomes from infected and uninfected human decidual immune cells samples. The decidual immune cells from 105 human voluntary abortion tissues were purified, and of the 5510 unique proteins identified, 181 proteins were found to be differentially abundant (>1.2-fold cutoff, P＜0.05) in the T. gondii-infected decidual immune cells. 11 proteins of 181 differentially expressed proteins associated with trophoblast invasion, placental development, intrauterine fetal growth, and immune tolerance were verified using a quantitative real-time polymerase chain reaction and western blotting. This systematic research identified a broad range of immune factors in human decidual immune cells, shedding a new insight into the decidual immune molecular mechanism for abnormal pregnancy outcomes associated with T. gondii infection.
2018-07-27 | PXD008448 | Pride
Project description:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone whose urinary (u-hCG) and recombinant (r-hCG) forms differ in glycosylation and stability. To test whether this reflects a structural reorganization of the α/β subunit interface, the two forms were compared by chemical crosslinking mass spectrometry (XL-MS). Crosslinking was performed on the intact, natively glycosylated heterodimers using two complementary chemistries: the lysine-reactive reagent DSS and the zero-length coupling reagent DMTMM (targeting Lys-Asp/Glu pairs, each in biological triplicate for both forms. Identified inter-subunit crosslinks were mapped onto the hCG structure to evaluate whether the assembled core interface is conserved between u-hCG and r-hCG. The dataset comprises the raw LC-MS/MS files and the corresponding crosslink identifications for all conditions and replicates, and accompanies a broader native-MS, ion-mobility and collision-induced-unfolding study of the two hCG forms.
2026-07-15 | PXD080682 | Pride
Project description:To understand the regulation of cytotoxicity by decidual CD8+ T cells (CD8+ dT) at the maternal-fetal interface, gene expression analysis of CCR7-CD45RA- effector-memory CD8+ T cells isolated from peripheral blood of unrelated healthy controls and decidual tissue from first trimester (6-12 weeks) and term (>37 weeks) pregnancy was performed. Furthermore, first trimester decidual effector-memory CD8+ T cells were stimulated with anti-CD3/CD28 in the presence of IL-2 for 12 hours or 72 hours. RNA was isolated and gene expression profiles were generated by employing Affymetrix HG_U133_Plus 2 arrays on the Affymetrix Geneatlas system.
2017-12-19 | E-MTAB-5890 | biostudies-arrayexpress
Project description:Uterine macrophages are thought to play an important regulatory role at the maternal-fetal interface. We report here a unique gene expression pattern intrinsic of first trimester decidual monocytes/macrophages, but not of their blood counterparts. The micro-array data comprises approximately 14,000 genes. Some of the key findings were confirmed by real time PCR or secreted protein measurements. A large number of regulated genes were found to be functionally related to immunomodulation and tissue remodelling, corroborating polarization patterns of differentiated macrophages of M2 phenotype. These include M2 markers such as CCL-18, CD209, insulin-like growth factor (IGF)-1, mannose receptor c type (MRC)-1 and fibronectin-1. Further, the selective up-regulation of triggering receptor expressed on myeloid cells (TREM)-2, alpha-2-macroglobulin (A2M) and prostaglandin D2 synthase (PGDS) provides new insights into the regulatory function of decidual macrophages in pregnancy. In addition, a large number of regulated genes in the micro-array analysis were related to cell cycle regulation. Taken together, molecular characterization of decidual macrophages presents a unique transcriptional profile replete with important components for fetal immunoprotection. Experiment Overall Design: Comparative expression analyis of CD14+ cells isolated either from blood or decidua. Decidual tissue and blood was obtained from eleven women between 18 and 41 years of age
2008-06-20 | E-GEOD-10612 | biostudies-arrayexpress