Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:Compared to other fish models, miRNAs are currently most extensively studied and identified in zebrafish. Approximately 415 dre-miRNAs have been identified and several articles have studied some aspect of miRNA function in zebrafish such as their role in basic development and in disease pathways. However, this field of research is in its infancy and the function of several dre-miRNAs, as well as their tissue-specific expression profile, are yet to be defined. In this study, the liver and gut were dissected (wildtype/untreated fish), total and small RNA were extracted, mRNA and miRNA libraries constructed and subjected to high throughput sequencing (HTS) using standard approaches. We carried out differential expression (DE) analysis and compared liver miRNA expression to gut using established bioinformatics pipelines. Through bioinformatics analysis, known and putative novel miRNAs were identified. Finally, we constructed a “miRNA matrix” that connects both total RNA-Seq and miRNA-Seq.
Project description:Compared to other fish models, miRNAs are currently most extensively studied and identified in zebrafish. Approximately 415 dre-miRNAs have been identified and several articles have studied some aspect of miRNA function in zebrafish such as their role in basic development and in disease pathways. However, this field of research is in its infancy and the function of several dre-miRNAs, as well as their tissue-specific expression profile, are yet to be defined. In this study, the liver and gut were dissected (wildtype/untreated fish), total and small RNA were extracted, mRNA and miRNA libraries constructed and subjected to high throughput sequencing (HTS) using standard approaches. We carried out differential expression (DE) analysis and compared liver miRNA expression to gut using established bioinformatics pipelines. Through bioinformatics analysis, known and putative novel miRNAs were identified. Finally, we constructed a “miRNA matrix” that connects both total RNA-Seq and miRNA-Seq.
