Project description:Expression profiling of WT and E2A-KO LSK FLT3- and LMPP protenitor cells. Experiment Overall Design: LSK FLT3- and LMPP stem/progenitor cells from WT and E2A-KO mice were FACS sorted. Subsequently RNA was extracted, labelled and hybridized to Affymetrix microarrays. Goal of experiment was to investigate expression changes between WT and KO LMPP cells.
Project description:We have utilized single-cell RNA sequencing to analyze how Alzheimer's disease mouse model is affecting the bone marrow hematopoiesis at the single-cell level. We sorted and analyzed the myeloid cells and the hematopoietic stem and progenitor cells from the bone marrow and myeloid cell from the brains of WT and 5xFAD mice. We also analyzed sorted myeloid cells from 5xFAD chimeric mice that had received a bone marrow transplant from WT or IFNAR1-KO donors.
Project description:High ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of hematopoietic stem cells (HSC) and are responsible for platelet formation. Using a mouse knockout model with normal megakaryocyte numbers but essentially devoid of LCM (MK-LCM KO), we demonstrated a pronounced increase in bone marrow HSC concurrent with endogenous mobilization and extramedullary hematopoiesis. When HSC isolated from a MK-LCM KO microenvironment were transplanted in lethally irradiated mice, the absence of LCM increased HSC in BM, blood and spleen. Severe thrombocytopenia was observed in animals with diminished LCM, although there was no change in megakaryocyte ploidy distribution. In contrast, WT HSC-generated LCM regulated a normal HSC pool and prevented thrombocytopenia. The present label-free quantitative LC-MSMS data was used to determine proteins that are differentially expressed in bone marrow cells of MK-LCM WT versus MK-LCM KO mice.
Project description:We investigated the contribution of NOX-2 to redox balance, mitochondrial respiration, and glycolytic compensation in lineage-negative Sca-1⁺c-Kit⁺ (SKL) bone marrow stem cells from wild-type (WT) and NOX-2 knockout (NOX2-KO) mice. Proteomic profiling identified differentially expressed proteins involved in hypoxic response, glycolysis, and oxidative stress adaptation between WT and NOX2-KO SKL cells.
