Project description:Chinese cordyceps is of particular interest for its confined distribution, mysterious lifecycle, ecological importance and developmental biology. The large scale artificial cultivation of this fungus has been succeeded in China until recently but with low efficiency and high cost being ascribed to too much unsolved biological issues, such as gene expression during development and the sexuality reproduction. The success of artificial cultivation provides the convenient for sampling during the different development stages.
Project description:Ophiocordyceps sinensis (Berk.), syn. Cordyceps sinensis, belongs to Ascomycetes and has been used as a traditional Chinese Medicine (TCM) in Asia countries over 2,000 y. It naturally inhabits in the Qinghai-Tibetan Plateau with an altitude of 3,000-5,000 m. Till now, over 20 bioactive ingredients exert over 30 activities. Several active peptides have been discovered from both the prokaryotes and eukaryotes. To reveal the differential protein profiles in different growth stages and ‘between the artificial one and the wild one’ , here the proteome data of the three adjacent growth stages and wild one were sequenced by using iTRAQ.
