Project description:The evolutionarily conserved trade-off between pathogen resistance and crop yield remains a major bottleneck in agricultural breeding. Although numerous genes governing disease resistance or growth productivity have been identified, the pleiotropic regulatory factors that harmonize these two agronomic traits remain largely elusive. Here, we report that the chloroplast oxygen-evolving complex protein GhPsbO is dually modulated at both translational and transcriptional levels by the Verticillium dahliae effector Vd10375. Overexpression of GhPsbO enhances photosynthesis and accelerates lignin deposition to establish structural defense, thereby simultaneously improving crop yield and disease resistance in cotton. Unexpectedly, knockout of GhPsbO enhanced resistance to V. dahliae by elevating chloroplast-derived reactive oxygen accumulation and result in plant sacrifices. Consistently, transcriptional repression of GhPsbO by GhMYB44 overexpression (OE) significantly improved cotton resistance, mirroring the GhPsbO knockout disease-resistant phenotype. Collectively, our findings uncover a novel regulatory module balancing plant growth and immunity, and provide a promising molecular strategy for breaking the growth-defense trade-off to achieve sustainable crop production.
Project description:Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene-specific level. To understand the post-transcriptional regulation during Drosophila early embryonic development, we used the sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs. A paper was published in Genome Biology 2007 under the tile Global analyses of mRNA translational control during early Drosophila embryogenesis". Experiment Overall Design: In this study, we have fractionated embryo extracts from a series of early stages by sedimentation on sucrose density gradients and analyzed the RNA components of these fractions. Our analysis has focused on analyzing ribosomal density, generally and for individual transcripts, global translational activity during the first 10 hours after egg laying and coordination between transcription and translation regulation.
Project description:gnp_blan06_torpten - rnai tor time course - The impact of the TOR pathway on growth and stress responses. Modification of translational and transcriptional profiles in Tor-inducible RNAi mutants and identification of TOR targets. 28 dye-swaps - normal vs. rnai mutant comparison.
Project description:Transcriptional profiling of human T cells analyzing the impact of race on the responsiveness to IFNa in healthy blood donors. Control and IFNa-treated samples derived from healthy Caucasian American vs. African American blood donors are compared. Two-condition experiment, Control vs. IFNa-treated. Paired design; untreated and treated samples for each donor.
Project description:Transcriptional profiling of human T cells analyzing the impact of race on the responsiveness to IFNa in healthy blood donors. Control and IFNa-treated samples derived from healthy Caucasian American vs. African American blood donors are compared.
Project description:Protein post-translational modifications transmit signals in part by creating binding sites for effector molecules. This is especially true in epigenetic pathways where histone tails are heavily modified, resulting in the recruitment of molecules that can affect transcription. One such molecule, plant homeodomain finger protein 20 (PHF20), uses a Tudor domain to read dimethyl-lysine residues and is a known component of the MOF histone acetyltransferase protein complex, suggesting it plays a role in the crosstalk between lysine methylation and histone acetylation. We sought to investigate the biological role of PHF20 by generating a knockout mouse. Without PHF20, mice die shortly after birth and display a wide variety of phenotypes within the skeletal and hematopoietic systems. Mechanistically, PHF20 is not required for maintaining the global H4K16 acetylation levels, but instead works downstream in transcriptional regulation of MOF target genes. ChIP sequencing of H4K16ace ChIP DNA from PHF20 knockout and wild type cells using Illumina Solexa Genome Analyzer II single end sequencing protocol.
Project description:Interventions: experimental group :PD-1 Knockout Engineered T Cells
Primary outcome(s): Number of participants with Adverse Events and/or Dose Limiting Toxicities as a Measure of Safety and tolerability of dose of PD-1 Knockout T cells using Common Terminology Criteria for Adverse Events (CTCAE v4.0) in patients
Study Design: historical control
Project description:We report genome-wide characterization of GATA3 binding sites in eleven well-defined developmental and effector cell types of the T lymphocyte lineage. By utilizing a conditional allele of GATA3, we investigated the impact of GATA3 expression on the mRNA expression patterns in several of these cell types. Correlation of GATA3 binding with gene expression changes indicates that GATA3 regulates a large number of stage- and cell-specific genes involved in multiple signaling and transcriptional pathways critical for T cell differentiation and immune responses. 1) RNA-Seq: Examination of RNA transcript levels in 6 cell types in wild-type and Gata3-knockout cells. 2) ChIP-Seq: Examination of GATA3 binding sites in 11 cell types. 3) Methyl-Seq: Examination of histone modification levels in DP cells from wild-type and Gata3-knockout mice.
Project description:gnp_blan06_torpten - rnai tor time course - The impact of the TOR pathway on growth and stress responses. Modification of translational and transcriptional profiles in Tor-inducible RNAi mutants and identification of TOR targets.
