Project description:RNA-Seq analyses were performed on GC-1 cells transfected with si-NC (control), si-Senp8, or si-Stat1, to study the regulatory networks between Senp8 and Stat1.
Project description:We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes.
Project description:Purpose: to find the potential down-stream target gene of circFAM120A Methods: mRNA profiles of decidualized human endometrial stromal cells (hESCs) after down-regulated FAM120A and control using siRNA were generated by NGS and compared by bioinformatics analysis Results: we sequenced 26414 mRNAs in hESCs between the si-NC and si-circFAM120A groups and identified 242 differentially expressed mRNAs, between which 161 were downregulated and 81 were up-regulated in si-circFAM120A group Conclusions: Our study represents the detailed analysis of decidualized hESCs transcriptomes between si-NC and si-circFAM120A groups.
Project description:In this project, the impact of subinhibitory concentrations of the fluoroquinolone ciprofloxacin on the multiresistant bacterium Stenotrophomonas maltophilia (strain LMG 26824) has been investigated. A label free proteomics analysis was performed to analyse the proteome of cells incubated with ciprofloxacin compared to a control sample.
