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Transcriptomic Analysis of Cortical and Medullary Thick Ascending Limb of Henle's loop (CTAL and MTAL) from WT or Cldn-10 Knock-out mice

ABSTRACT: Transcriptomic Analysis of Cortical and Medullary Thick Ascending Limb of Henle's loop (CTAL and MTAL) from WT or Cldn-10 Knock-out mice

PROVIDER: PRJNA1062457 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27462414_1.fastq.gz	Fastqsanger.gz
	SRR27462414_2.fastq.gz	Fastqsanger.gz
	SRR27462415_1.fastq.gz	Fastqsanger.gz
	SRR27462415_2.fastq.gz	Fastqsanger.gz
	SRR27462416_1.fastq.gz	Fastqsanger.gz

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Profiling of Transcripts in Rat Kidney Medullary Thick Ascending Limb

Project description:Freshly isolated rat kidney medullary thick ascending limbs were subjected for transcript profiling. Three microarray experiments were done to obtain the kidney medullary thick ascending limb transcriptome.

2009-01-26 | E-GEOD-13669 | biostudies-arrayexpress

Profiling of Transcripts in Rat Kidney Medullary Thick Ascending Limb

Project description:Freshly isolated rat kidney medullary thick ascending limbs were subjected for transcript profiling.

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snRNAseq of mouse thick ascending limb cells

Project description:Kidney thick ascending limb (TAL) cells display a remarkable amount of regional and functional heterogeneity. We applied single-nucleus RNA-seq to map the molecular heterogeneity of enriched Slc12a1+ cells.

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snRNAseq of mouse thick ascending limb cells

Project description:snRNAseq of mouse thick ascending limb cells

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Transcriptomic Analysis of Cortical and Medullary Thick Ascending Limb of Henle's loop (CTAL and MTAL) from WT or Cldn-10 Knock-out mice

Project description:We applied RNAseq analysis on microdissected cortical and medullary thick ascendind limb of Henle’s loop from WT and Cldn10 KO mice (cKO). Among 11369 genes sequenced 291 have an expression significantly modified (p<0.05) between MTAL and CTAL from WT mice. In cKO mice, among 12023 genes sequenced 359 were signicantly modified (p<0.05) between MTAL and CTAL. surprisingly, while 637 genes have significantly (p<0.05) different expression between MTAL WT and cKO only 66 were in this case between CTAL WT and cKO.

2024-02-01 | GSE252783 | GEO

Project description:We report the relative difference in medullary thick ascending limb (mTAL) transcript between SS/JrHsdMcwi and consomic SS-Chr 1BN/Mcwi (SS.BN1) rats.

2022-05-21 | GSE203266 | GEO

Rattus norvegicus

Project description:Profiling of Transcripts in Rat Kidney Medullary Thick Ascending Limb

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Expression and function of NFAT5 in medullary thick ascending limb (mTAL) cells.

Project description:The contribution of nuclear factor of activated T cells 5 (NFAT5) to the regulation of tumor necrosis factor-alpha (TNF) production in medullary thick ascending limb (mTAL) cells is unclear. RT-PCR analysis was performed on primary cultures of mouse mTAL cells and freshly isolated mTAL tubules to determine which NFAT isoforms are present in this nephron segment. Primer pairs were designed, based on published sequences for mouse NFAT1-5, to produce fragments of approximately 200 bp. Analysis of PCR products by gel electrophoresis and subsequent DNA sequencing indicated that cells and tubules contained mRNA for all five NFAT isoforms. The relative expression of NFAT isoforms was then determined using quantitative real-time RT-PCR. The data indicate that NFAT isoforms 5 >/= 1 are the predominant isoforms present in mTAL cells and tubules. Western blot analysis demonstrated constitutive expression of NFAT5 in nuclear extracts from mTAL tubules and primary culture cells; expression in mTAL cells also was detected by immunofluorescence. Expression of NFAT5 was increased in mTAL cells transiently transfected with an NFAT5 overexpression vector (pcDNA3.1-NFAT5), resulting in increased basal and calcium-sensing receptor (CaR)-mediated TNF production. Transient transfection of mTAL cells with a small hairpin RNA vector that targeted exon 8 of NFAT5 (U6-N5 ex8) significantly inhibited TNF promoter activity. Transient transfection with U6-N5 ex8 also reduced nuclear expression of NFAT5, TNF mRNA accumulation, and attenuated CaR-mediated activation of Cl(-) entry into polarized mTAL cells. Collectively, these data suggest that activation of NFAT5 is part of a TNF-dependent pathway that inhibits apical Cl(-) influx in the mTAL after activation of CaR.

| S-EPMC2692445 | biostudies-literature

Increased proliferative cells in the medullary thick ascending limb of the loop of Henle in the Dahl salt-sensitive rat.

Project description:Studies of transcriptome profiles have provided new insights into mechanisms underlying the development of hypertension. Cell type heterogeneity in tissue samples, however, has been a significant hindrance in these studies. We performed a transcriptome analysis in medullary thick ascending limbs of the loop of Henle isolated from Dahl salt-sensitive rats. Genes differentially expressed between Dahl salt-sensitive rats and salt-insensitive consomic SS.13(BN) rats on either 0.4% or 7 days of 8.0% NaCl diet (n=4) were highly enriched for genes located on chromosome 13, the chromosome substituted in the SS.13(BN) rat. A pathway involving cell proliferation and cell cycle regulation was identified as one of the most highly ranked pathways based on differentially expressed genes and by a Bayesian model analysis. Immunofluorescent analysis indicated that just 1 week of a high-salt diet resulted in a severalfold increase in proliferative medullary thick ascending limb cells in both rat strains, and that Dahl salt-sensitive rats exhibited a significantly greater proportion of medullary thick ascending limb cells in a proliferative state than in SS.13(BN) rats (15.0±1.4% versus 10.1±0.6%; n=7-9; P<0.05). The total number of cells per medullary thick ascending limb section analyzed was not different between the 2 strains. The study revealed alterations in regulatory pathways in Dahl salt-sensitive rats in tissues highly enriched for a single cell type, leading to the unexpected finding of a greater increase in the number of proliferative medullary thick ascending limb cells in Dahl salt-sensitive rats on a high-salt diet.

| S-EPMC3760736 | biostudies-literature

Medullary thick ascending limb buffer vasoconstriction of renal outer-medullary vasa recta in salt-resistant but not salt-sensitive rats.

Project description:We have demonstrated previously that paracrine signaling occurs between medullary thick ascending limb (mTAL) and the contractile pericytes of outer-medullary vasa recta (VR), termed "tubular-vascular cross-talk." The aim of the current study was to determine whether tubular-vascular cross-talk has a functional effect on vasoconstrictor responses to angiotensin II and to determine whether this is altered in the Dahl salt-sensitive (SS) rat. Studies were performed on salt-resistant consomic SS.13 Brown Norway (BN) and SS rats using a novel outer medullary tissue strip preparation in which freshly isolated VRs within VR bundles were perfused either alone or in combination with nearby mTAL. In VRs from SS.13BN rats, angiotensin II (1 µmol/L) increased VR bundle intracellular Ca2+ concentration 19±9 nmol/L (n=8) and reduced focal diameter in perfused VRs by -20±7% (n=5). In the presence of nearby mTAL, however, VR bundle intracellular Ca2+ concentration (-9±8 nmol/L; n=8) and VR diameter (-1±4%, n=7) in SS.13BN rats were unchanged by angiotensin II. In contrast, in Dahl SS rats, angiotensin II resulted in rapid and sustained increase in VR bundle intracellular Ca2+ concentration (89±48 nmol/L, n=7; 50±24%, n=8) and a reduction in VR diameter of (-17±7%, n=7; -11±4%, n=5) in both isolated VRs and VRs with nearby mTAL, respectively. In VRs with mTAL from SS13BN rats, inhibiton of purinergic receptors resulted in an increase in VR bundle intracellular Ca2+ concentration, indicating that purinergic signaling buffers vasoconstriction. Importantly, our in vitro data were able to predict medullary blood flow responses to angiotensin II in SS and SS.13BN rats in vivo. We conclude that paracrine signaling from mTAL buffers angiotensin II vasoconstriction in Dahl salt-resistant SS.13BN rats but not SS rats.

| S-EPMC3785299 | biostudies-literature

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